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Plasmid#78540PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMammalianMutationPromoterU6 and U6 (for expressing sgRNA)Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJA2
Plasmid#19465DepositorInsertDnaJ subfamily A member 2 (DNAJA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-BICD2N
Plasmid#64070PurposeN-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)DepositorInsertBICD2N (1-400) (Bicd2 Mouse)
UseTagsGFP and His-ZZ-TCSExpressionInsectMutationCodon optimized for Sf9 expressionPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
XW17
Plasmid#65835PurposePunc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsmCherryExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJA3
Plasmid#19547DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
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E1b-GFP-Tol2-Gateway dre SE-zic2a P
Plasmid#89385PurposeVector for reporter assay of one zebrafish super-enhancer regionDepositorInsertzic2a (zic2a Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX ShcA-SH2
Plasmid#46486DepositorInsertShcA (SHC1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 476-584PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PD-L1-miSFIT-4x
Plasmid#124684PurposeTuning expression of PD-1DepositorInsertPD-L1 (CD274 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMBEC8
Plasmid#187599PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
[pSK475] pLJC5 HA-Depdc5(A)
Plasmid#109344PurposeLenti-viral expression of HA-tagged Depdc5 mutant ADepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsHAExpressionMutationDIYGD(185-189)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK476] pLJC5 HA-Depdc5(B)
Plasmid#109345PurposeLenti-viral expression of HA-tagged Depdc5 mutant BDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsHAExpressionMutationEQPLH(371-375)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK478] pLJC5 HA-Depdc5(P)
Plasmid#109347PurposeLenti-viral expression of HA-tagged Depdc5 mutant PDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsHAExpressionMutationYDLLP(775-779)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
LN-entry
Plasmid#128012PurposeVector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag
UseLamdan entry vectorTagsLN-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
px330 MAU2 gRNA
Plasmid#165589PurposeInsertion of MAU2 degronDepositorInsertMAU2 (CTCF Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nck-SH2
Plasmid#46457DepositorInsertNck (NCK1 Human)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 275-377PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
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VEGF binding scFv G6
Plasmid#111716PurposeYeast Surface Display of G6 as a reference starting point for designDepositorInsertG6
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-GFP11
Plasmid#61670PurposeNon-fluorescent, truncated kinesin-1 fused to split GFP linkerDepositorInsertKIF5C(1-560)-GFP11 (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-mCitrine
Plasmid#61676PurposeSingle mCitrine-labeled, truncated kinesin-1DepositorInsertKHC(1-560)-mCitrine (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only