Addgene

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pTRANS_101

Plasmid

#91199

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9C-P2A-turboGFP

Plasmid

#80935

Purpose

Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2

Plasmid

#61260

Purpose

Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Use

Tags

Expression

Worm

Mutation

Promoter

heat shock promoter

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA1

Plasmid

#61259

Purpose

Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA1

Use

Tags

Expression

Worm

Mutation

Promoter

heat shock promoter

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET30-T7-sfGFP

Plasmid

#180757

Purpose

pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Use

Tags

strep tag

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 RAD54B

Plasmid

#67124

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RAD54B (RAD54B Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pMM0530

Plasmid

#128987

Purpose

Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-ZiF268DBD-CRY2PHR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDM1501

Plasmid

#108997

Purpose

safe haven knock-in plasmid for the act5 locus

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus

Tags

Expression

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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mEGFP-4xbiRhoBAST

Plasmid

#196350

Purpose

Expression of mEGFP mRNA with 4xbiRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-4xbiRhoBAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMX1418

Plasmid

#127856

Purpose

Expresses EGFP-IPNN-MCAK in mammalian cells

Depositor

Linda Wordeman

Article

Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. d

Insert

IPNN-MCAK

Use

Tags

EGFP

Expression

Mammalian

Mutation

mutation is I97N and P98N

Promoter

CMV

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMC-1-PvUbi2-6

Plasmid

#197706

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PvUbi2 promoter

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.4_mCherry-MMLV_Psi

Plasmid

#205522

Purpose

Express mCherry-Psi (export tag consisting of MMLV Psi)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#197700

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtUbi10 promoter

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-dSa-VP64

Plasmid

#99680

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VP64

Expression

Mutation

dead Cas9

Promoter

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta13

Plasmid

#13559

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pDM1208

Plasmid

#108985

Purpose

extrachromosomal expression vector, N-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector

Tags

mCherry

Expression

Mutation

Promoter

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTCW007

Plasmid

#83525

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha1

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1J2

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pTRANS_233

Plasmid

#91122

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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RAB14 Q70L

Plasmid

#61494

Purpose

to overexpress constitutively active RAB14. Vector includes GFP driven by hUbC promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral

Tags

Expression

Mutation

Q70L, active form

Promoter

EF1a

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only