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264,707 results

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pBabe puro HA PIK3CA

Plasmid

#12522

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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CellTagA

Plasmid

#124591

Purpose

Expressed barcode for sample multiplexing in single-cell analysis

Depositor

Samantha Morris

Article

Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

Insert

CellTagA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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CellTagA

Plasmid

#124591

Purpose

Expressed barcode for sample multiplexing in single-cell analysis

Depositor

Samantha Morris

Article

Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

Insert

CellTagA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSG4K5

Plasmid

#74492

Purpose

For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSG4K5

Plasmid

#74492

Purpose

For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Tags

Expression

Mutation

Promoter

L-arabinose-inducible promoter PBAD

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF3005

Plasmid

#144481

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3005

Plasmid

#144481

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-STAT3

Plasmid

#111934

Purpose

Expresses human STAT3 fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Signal transducer and activator of transcription 3 (STAT3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-STAT3

Plasmid

#111934

Purpose

Expresses human STAT3 fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Signal transducer and activator of transcription 3 (STAT3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MEF2C-HA

Plasmid

#32515

Depositor

Andrew Lassar

Article

Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

Insert

Mef2c (Mef2c Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MEF2C-HA

Plasmid

#32515

Depositor

Andrew Lassar

Article

Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

Insert

Mef2c (Mef2c Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#159281

Purpose

A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

AsCpf1-HA-2A-GFP

Use

Multicistronic vector with both caggs promoter-dr…

Tags

3X HA

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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