Addgene

We narrowed to 18,521 results for: omp

Showing: 8881 - 8900 of 18521 results

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pET30a-SARS-CoV2-ORF8

Plasmid

#165089

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-EGFP-MACROD1

Plasmid

#172584

Purpose

For transient expression of N-terminal GFP-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Mau2_BSD_FKBPF36V_EYFP

Plasmid

#163880

Purpose

Acute Depletion of Mau2-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Mau2 (Mau2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-cdc42palm

Plasmid

#20141

Depositor

Alaa El-Husseini

Article

Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

Insert

cell division cycle 42 isoform 2 (CDC42 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

wild type

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
CAG-eYFP-3x-miR708-5p-TS

Plasmid

#117381

Purpose

An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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ZIKV_Trigger_32B

Plasmid

#75009

Purpose

Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

Depositor

Alexander Green, James Collins

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_32B

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 GFP-PNRC1

Plasmid

#123295

Purpose

Mammalian expression of PNRC1 N-GFP

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_TP53BP2-1/1

Plasmid

#91503

Purpose

Protein expression and purification of human SH3 domain construct TP53BP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TP53BP2-1/1 (TP53BP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SASH3-1/1

Plasmid

#91434

Purpose

Protein expression and purification of human SH3 domain construct SASH3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SASH3-1/1 (SASH3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_YES1-1/1

Plasmid

#91439

Purpose

Protein expression and purification of human SH3 domain construct YES1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

YES1-1/1 (YES1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-MACROD2

Plasmid

#172585

Purpose

For transient expression of N-terminal GFP-tagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 UBXN11

Plasmid

#169017

Purpose

Expresses GST-UBXN11 (human) in bacteria

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN11 (UBXN11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133-mut

Plasmid

#87339

Purpose

To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes; deletion of Nup133 AA1-7

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187918

Purpose

Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 1

Use

Tags

3xFLAG

Expression

Mutation

Promoter

CMV Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp5

Plasmid

#168954

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp5 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp5

Plasmid

#168933

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp5 (ORF1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCasper-pNos-PCP-eGFP

Plasmid

#180274

Purpose

to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

PCP-eGFP

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

nanos

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-Cdh1

Plasmid

#28127

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

fzr1 (FZR1 Human)

Use

Mouse Targeting and Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
PVT1-luciferase

Plasmid

#113350

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pfastbacHTb/mClock26-384

Plasmid

#47336

Purpose

mClock fragment cloned into pfastbacHTb vector with Hexa His tag

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

His and Tev

Expression

Mutation

aa 26-384

Promoter

PH

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only