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Plasmid#191860PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only
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pCRISPR3_BC4
Plasmid#191859PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC3
Plasmid#191858PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC1
Plasmid#191856PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-panTRK-CR1-EGFP
Plasmid#187194PurposeTo express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3DepositorInsertCR1 pan-TRK receptor
UseRetroviralTagsEGFPExpressionMammalianMutationPromotermo-MLV or MMLVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP728_L2_pV01_0I_FRT-TB-rLUC_TCTP-fLUC
Plasmid#192371PurposeTo test for the repressive potential of the Transcription Block (TB) with the FRT sites around it.DepositorInsertAct2::FRT-TB-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hygro-Bem1-mCherry-AID
Plasmid#173925PurposePlasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AIDDepositorInsertsPartial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence
UseTagsmCherry- AIDExpressionMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MON +T111H
Plasmid#187432PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged threonine at 117 to histidinePromoterPH and p10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET41c-PhTET2
Plasmid#182428PurposeExpresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coliDepositorInsertTetrahedral aminopeptidase TET2 from P. hirokoshii
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_UGI-NES_right
Plasmid#187846Purposeexpresses the right-side half ND6-UGI-NES in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_UGI-NES_left
Plasmid#187845Purposeexpresses the left-side half ND6-UGI-NES in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSK573
Plasmid#190881PurposeFor C-terminal -Halo Tagging of a gene of interest with TRP1 selection marker in yeast S. cerevisiaeDepositorInsertHaloTag
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
TEG +H122D
Plasmid#187434PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 128 to aspartic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H111E
Plasmid#187429PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 117 to glutamic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KEE +E111H
Plasmid#187431PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged glutamic acid at 117 to histidinePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNG +R111Q
Plasmid#187437PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged arginine at 117 to glutaminePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H122D
Plasmid#187428PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 128 to aspartic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
MON +N122H
Plasmid#187433PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged asparagine at 128 to histidinePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
OST +Q111R
Plasmid#187436PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged glutamine at 116 to argininePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H111T
Plasmid#187430PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 117 to threoninePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only