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Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
UseTagsMPBExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV
Plasmid#24828DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSExpressionMutationPromoterTRE, PGKAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBwt-PURO
Plasmid#208045PurposeEnables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycinDepositorInsertUb (UBC Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationPromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pGP-AAV-syn-jGCaMP7b-WPRE (AAV Retrograde)
Viral Prep#104489-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP7b-WPRE (#104489). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7b-WPRE plasmid DNA. Syn-driven jGCaMP7b calcium sensor. jGCaMP7b exhibits the brightest resting fluorescence of the jGCaMP7 variants and can be used for imaging of small neuronal processes. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Trim46
Plasmid#176402PurposeTRIM46 tagged with GFPDepositorInserttrim46 (Trim46 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK- dCas9-SunTag-P2A-HygR
Plasmid#193137PurposeExpresses dCas9 fused to the SunTag scaffoldDepositorInsertdCas9-SunTag-T2A-HygR
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-GCaMP6s
Plasmid#135418PurposeCan be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.DepositorInsertGCaMP6s
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2.0VPI.EGFP
Plasmid#40868DepositorInsertmouse vasopressin promoter
UseTagsExpressionBacterial and InsectMutationPromotermouse vasopressinAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
mKeima-Red-N1
Plasmid#54597PurposeLocalization: N1 Cloning Vector, Excitation: 440, Emission: 620DepositorTypeEmpty backboneUseTagsmKeima-RedExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMARO
Plasmid#178086PurposeDerivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.DepositorInsertTcR (tetA/tetR)
UseTagsExpressionBacterialMutationTetracycline resistance cassette is flanked by Ec…PromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-N1
Plasmid#54843PurposeLocalization: N1 Cloning Vector, Excitation: 434, Emission: 474DepositorTypeEmpty backboneUseTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-FLAG-Luc-N
Plasmid#58790PurposeExpress LucN alone in mammalian cellsDepositorInsertfirefly Luciferase 1-398
UseTagsFLAGExpressionMammalianMutationT576C,T591C to destroy MfeI and EcoRI sitesPromoterCMVAvailable sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Luc-C
Plasmid#58791PurposeExpress LucC alone in mammalian cellsDepositorInsertfirefly Luciferase 394-550
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mGreenLantern
Plasmid#164459PurposeLocalization of mGreenLantern fluorescent protein to actinDepositorInsertmGreenLantern
UseTagsLifeActExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMPE-ePPEplus
Plasmid#205242PurposeFor multiplex plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_EGFP
Plasmid#183117PurposeExpresses flag-tagged EGFP in mammalian cellsDepositorInsertEGFP
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X
Plasmid#192151PurposeT cell CRISPR AAV-SB vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only