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Plasmid#120075PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-TagDepositorInsertS-Tag
UsePcr templateTagsS-TagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z30
Plasmid#120073PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-GDepositorInsertVSV-G
UsePcr templateTagsVSV-GExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Theo-LUCID
Plasmid#119048PurposePlasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Theo-LUCID_part1
Fab_Theo-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Quin-LUCID
Plasmid#119049PurposePlasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Quin-LUCID_part1
Fab_Quin-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_MTX-LUCID
Plasmid#119047PurposePlasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_MTX-LUCID_part1
Fab_MTX-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP
Plasmid#114730PurposesgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M7
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7T3-PacI_MafB_ISH
Plasmid#87670PurposeIn situ probe for mouse MafbDepositorInsertMafb (Mafb Mouse)
UseTagsExpressionBacterialMutationPromoterT7/T3Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-PdhR
Plasmid#89655PurposePlasmid for titrating synthesis of PdhR with doxycyclineDepositorInsertPyruvate dehydrogenase complex repressor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsB
Plasmid#107559PurposePlasmid for titrating synthesis of FtsB with doxycyclineDepositorInsertftsB
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Tam1
Plasmid#101639PurposeEncodes the fluorescent protein Tam1 for arabinose-inducible expression in E. coli.DepositorInsertTam1
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pThom77.6
Plasmid#101637PurposeEncodes the fluorescent protein amFP486/K68M under a CMV promoter.DepositorInsertamFP486/K68M
UseTagsExpressionMammalianMutationK68MPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pThom77.3
Plasmid#101636PurposeEncodes the fluorescent protein amFP486/K68M for constitutive expression in E. coli.DepositorInsertamFP486/K68M
UseTags6XHisExpressionBacterialMutationK68MPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Epsilon (JCE592)
Plasmid#89776Purposeyeast expression of mouse PKC Epsilon with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Epsilon (Prkce Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCm7RNA6A
Plasmid#84367PurposeCas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockinDepositorInsertCbfa2t2 m7-gRNA6
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCBFKOgRNA5
Plasmid#84366PurposeCas9-gRNA plasmid for mouse Cbfa2t2 KnockoutDepositorInsertCbfa2t2-gRNA5
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCBFKOgRNA2
Plasmid#84365PurposeCas9-gRNA plasmid for mouse Cbfa2t2 KnockoutDepositorInsertCbfa2t2-gRNA2
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-Mst2 SARAH
Plasmid#84298PurposeExpresses Mst2 SARAH domain for interaction and spectroscopy studies.DepositorInsertMst2 (STK3 Human)
UseTagsGSTExpressionBacterialMutationL443WPromotertacAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFusX4-41
Plasmid#63549PurposeEncodes RVD positions 10-12 in TALEN assemblyDepositorInsertNN NN NI
UseTALENTagsExpressionMutationnonePromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFusX4-62
Plasmid#63550PurposeEncodes RVD positions 10-12 in TALEN assemblyDepositorInsertNG NG HD
UseTALENTagsExpressionMutationnonePromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFusX4-63
Plasmid#63551PurposeEncodes RVD positions 10-12 in TALEN assemblyDepositorInsertNG NG NN
UseTALENTagsExpressionMutationnonePromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only