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Plasmid#111718PurposeYeast Surface Display of D44.1 as a reference starting point for designDepositorInsertD44.1
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-p97-RH-mycStrep
Plasmid#31839DepositorInsertp97
UseTagsStrep and mycExpressionBacterial and MammalianMutationR155HPromoterCMVAvailable sinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVL94 - pOp4:VND7
Plasmid#116016Purposedestination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-inductionDepositorInsertpOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
49535-sgFMR1_E3B
Plasmid#157783PurposeExpresses a sgRNA targeting the 3rd exon of human FMR1 geneDepositorInsertFMR1 (FMR1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
cpGFP-Adhiron_flex
Plasmid#165044PurposeFlexible linker control for POLArISact expression in mammalian cellsDepositorInsertcpGFP-Adhiron (connected with a flexible linker)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-NEMO-C54/347A
Plasmid#27268DepositorInsertNEMO (IKBKG Human)
UseTagsFLAGExpressionMammalianMutationCys54Ala Cys347AlaPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-DR-cr in PBCSK-
Plasmid#126642PurposeCloning vector FnoCas12a DR-crRNA for expression in mammalian cellsDepositorInsertFnoCas12a Full Length Direct Repeat crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Emerin
Plasmid#120246PurposeExpresses V5-tagged Emerin in lentiviral vectorDepositorInsertEmerin (Emd Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe_PepTSo
Plasmid#58334PurposeExpresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTso
UseTagsGFP-HisExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV6:T35s
Plasmid#164741PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr3g095180.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV2:T35s
Plasmid#164740PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr5g012400.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
ins:MCS2; cryaa:RFP
Plasmid#110286PurposeA zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.DepositorTypeEmpty backboneUseZebrafishTagsExpressionMutationPromoterinsulinAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET19b-hMED9
Plasmid#15365DepositorInserthMED9 (MED9 Human)
UseTagsHisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
MO70-hJunctate - EF-mutant
Plasmid#79597PurposeTransient and retroviral expressionDepositorInsertjunctate (ASPH Human)
UseRetroviralTagsFLAGExpressionMammalianMutation77DAD79 to 77AAA79PromoterCMVAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-CT
Plasmid#64063Purposemyc-tagged murine seipin (cytosolic C-terminus); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationContains Arg326 to Ser443PromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-p97-RG-mycStrep
Plasmid#31840DepositorInsertp97
UseTagsStrep and mycExpressionBacterial and MammalianMutationR95GPromoterCMVAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2VL-IRES-dOrai-IRES-GFP
Plasmid#72897PurposeMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2VL-IRES-dOrai-IRES-GFP
UseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/G51PEKN
Plasmid#118583PurposeBacterial expression of E.coli DHFR N23PP and G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23, G51 mutated to P51, a Pro res…PromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
4E2H.210_LUMABS
Plasmid#155209PurposeExpresses 4E2H.210_LUMABS sensor in E.coliDepositorInsert4E2H.210_LUMABS
UseTags6x His TagExpressionMutationPromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only