Addgene

We narrowed to 23,927 results for: pET

Showing: 8801 - 8820 of 23927 results

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pGEX-2TK cyclin T1 (1-300) (P#432)

Plasmid

#14608

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-300) (CCNT1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-300

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
gZiPro

Plasmid

#198441

Purpose

Activity/inhibition assays, 19F-NMR

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A,

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG wildtype, catalytic dead lipin 1

Plasmid

#32006

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D712E and D714E

Promoter

CMV, SP6

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
plentiCRISPv2_AAVS1

Plasmid

#184487

Purpose

Lentivirus all in one CRISPR vector targeting human AAVS1 gene

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

AAVS1 (AAVS1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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PLEX_313 SLC31A1

Plasmid

#184493

Purpose

Lentivirus expression for SLC31A1 gene

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

SLC31A1 (SLC31A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-puro FLAG wildtype, catalytic active lipin 1

Plasmid

#32010

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pSV CDK9 (D167N) HA (P#601)

Plasmid

#14639

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (1-250) (P#467)

Plasmid

#14606

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-250) (CCNT1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-250

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pLINK CDK9 myc (D167N) (P#1084)

Plasmid

#14637

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK cyclin T1 (1-551) (P#422)

Plasmid

#14609

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-551) (CCNT1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-551

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pEF myc Gal CDK9 (P#1475)

Plasmid

#14631

Depositor

Matija Peterlin

Article

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

cdk9 (CDK9 Human)

Use

Tags

Gal and myc

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmMextli

Plasmid

#79262

Purpose

Expresses EGFP-tagged DmMextli in S2 cells

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli (mxt Fly, NM_164598)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-Hs4E-BP3

Plasmid

#79439

Purpose

Expresses lambdaN-HA-tagged human 4E-BP3 in Mammalian cells

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4E-BP3 (EIF4EBP3 Human)

Use

Tags

lambdaN-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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Rev CDK9 (D167N) (P#858)

Plasmid

#14635

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Use

Tags

Rev

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
gZiPro W5F

Plasmid

#198443

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W5F

Promoter

T7 promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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5ZiPro

Plasmid

#198432

Purpose

Conformation sensitive site specific Cys-labeling

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

S84*C, R95*A, C80S, C143S, S160C, C178S,

Promoter

T7 promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W61*F

Plasmid

#198442

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W61*F, R95*A,

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro W5F

Plasmid

#198436

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W5F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro W83F

Plasmid

#198439

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W83F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
gZiPro W89F

Plasmid

#198447

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W89F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge