-
Plasmid#222216PurposeExpress DDX53 mutant Q249A protein in bacteriaDepositorInsertDDX53_Q249A (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationK272APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-K272A
Plasmid#222215PurposeExpress DDX53 mutant K272A protein in bacteriaDepositorInsertDDX53_K272A (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationK272APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-E377Q
Plasmid#222217PurposeExpress DDX53 mutant E377Q protein in bacteriaDepositorInsertDDX53_E377Q (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationE377QPromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I75_tUBQ10_GG5-GG6
Plasmid#219714PurposeLevel 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.DepositorInsertGG5-tUBQ10-GG6
UseSynthetic BiologyTagsExpressionMutationWT terminator with added golden gate linkersPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57_beta-globin-5'UTR_Nanoluc
Plasmid#219989Purposebetaglobin-5'UTR-NanoLuc mRNA synthesisDepositorInsertbetaglobin-5'UTR-NanoLuc (HBB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I89-pMUTE_GG0-1
Plasmid#219708PurposeLevel 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pMUTE-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-GST-MPK6
Plasmid#215170PurposeEncodes the glutathionine S-transferase with N-terminal His tag and fused to the N-terminus of a mitogen-activated protein kinase 6 from Arabidopsis thalianaDepositorInsertHis-GST-MPK6
UseTagsHis-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I74-_tUBQ10_GG4-GG6
Plasmid#219713PurposeLevel 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.DepositorInsertGG4-tUBQ10-GG6
UseSynthetic BiologyTagsExpressionMutationWT terminator with added golden gate linkersPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I87-SPCHPromoter_GG0-1
Plasmid#219707PurposeLevel 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pSPCH-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I80_Bxb1mut(noBsaI)_GG1-GG4
Plasmid#219715PurposeLevel 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.DepositorInsertGG1-Bxb1(noBsaI0)-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-TULP3
Plasmid#218741PurposeExpresses C-terminally EGFP-tagged TULP3 in mammalian cellsDepositorInsertTULP3 (TULP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vMAT1-VL-hIgG1-CL
Plasmid#218441Purposemammalian expression plasmid for the light chain fragment of an anti-vMAT1 Fab domainDepositorInsertvMAT1-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vGLUT1-VL-hIgG1-CL
Plasmid#218439Purposemammalian expression plasmid for the light chain fragment of an anti-vGLUT1 Fab domainDepositorInsertvGLUT1-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vMAT2-VL-hIgG1-CL
Plasmid#218443Purposemammalian expression plasmid for the light chain fragment of an anti-vMAT2 Fab domainDepositorInsertvMAT2-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:MCS+:NosT
Plasmid#212160PurposeThis binary vector has an empty backbone with extended multiple cloning site (MSC) where you can inserta a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35S[TMV]:mNeonGreen:NosT
Plasmid#212162PurposeThis binary vector expresses mNeonGreen with a truncated 35sCAMV promoter with strong UTR (Tomato Mosaic Virus)DepositorInsertTomato codon optimized version of mNeonGreen
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomatoPromotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RHOAA-c035
Plasmid#205118PurposeRHOA protein expressionDepositorInsertRHOA (RHOA Human)
UseTags6His-TEV and AviTagExpressionBacterialMutationWTPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K40R-short-UBASH3A-Ntag
Plasmid#195401PurposeHuman K40R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationK40RPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K40R-short-UBASH3A-Ctag
Plasmid#195400PurposeHuman K40R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationK40RPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfCherry11-Ex1-17-PTPN22
Plasmid#195387PurposeMammalian expression of a human PTPN22 mutant (exon 1-17) with sfCherry(11) tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagssfCherry(11)ExpressionMammalianMutationPTPN22 mutant containing exon 1 to 17PromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only