Addgene

We narrowed to 8,349 results for: pcDNA3

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pcDNA3.1 GW-mCit-PA

Plasmid

#113449

Purpose

ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

mCitrine-ProteinA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-PAFAH1B1_R22-

Plasmid

#92431

Purpose

mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).

Depositor

Matthias Leisegang, Ralf Brandes

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

Tags

Expression

Mammalian

Mutation

R22 (stop codon in position 22)

Promoter

CMV

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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tubulin-ReNL_pcDNA3

Plasmid

#89530

Purpose

Red color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-pHlash

Plasmid

#61552

Purpose

Expression of pHlash in mammalian cells

Depositor

Carl Johnson

Article

Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

Insert

pHlash

Use

Tags

NA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mGAT1

Plasmid

#22804

Depositor

Henry Lester

Article

Mager et al J Neurosci. 1996 Sep 1. 16(17):5405-14.

Insert

pcDNA3.1-mGAT1

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-hBIN1-V5

Plasmid

#27303

Depositor

Robin Shaw

Article

Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Insert

bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)_mtZFN(-)2G

Plasmid

#62799

Purpose

mtZFN2G architecture bearing (-) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(-)2G

Use

Tags

Internal FLAG tag immediately after F1B mitochond…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(+)2G

Plasmid

#62798

Purpose

mtZFN2G architecture bearing (+) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(+)2G

Use

Tags

Internal HA tag immediately after F1B mitochondri…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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NF-155 pcDNA3

Plasmid

#197290

Purpose

Plasmid expressing an antigen targeting the Plasmid expressing an antigen targeting Neurofascin-155

Depositor

Peter Brophy

Article

Collinson et al Glia. 1998 May;23(1):11-23

Insert

Pan-Neurofascin (extracellular) (Nfasc Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcdna3mciap1 mut

Plasmid

#11476

Depositor

Jon Ashwell

Article

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Insert

mciap1mut (Birc2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

582 to alanine

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CCND1

Plasmid

#172632

Purpose

Expresses untagged cyclin D1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-cT

Plasmid

#104355

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TRAIL-R4

Plasmid

#61385

Purpose

Expresses HA tagged TRAIL receptor 4

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 4 (TNFRSF10D Human)

Use

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R4 leader peptide (aa1-29) replaced with TN…

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+N-eGFP_SAMD9L_R986C

Plasmid

#206176

Purpose

mammalian expression of SAMD9L R986C

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

SAMD9L (SAMD9L Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R986C

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-GW

Plasmid

#113448

Purpose

ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

ProteinA-mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hPMCA4b

Plasmid

#47595

Depositor

Emanuel Strehler

Insert

PMCA4b (ATP2B4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-GI-ZFP2

Plasmid

#42215

Purpose

Expresses GI-ZFP2 for light-inducible activation of gene expression

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

GIGANTEA-FLAG-ZFP2 (GI Mustard Weed, Synthetic)

Use

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR(T/A)

Plasmid

#118465

Purpose

Negative-control mutant for sapphireAKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR(T/A)

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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