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Plasmid#195012Purposebleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+BLE+tGPD
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3
Plasmid#63199PurposeBioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV113.loxP
Plasmid#63186PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV115.loxP
Plasmid#63187PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV116.loxP
Plasmid#63188PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-NeoR-SV40 polyA-Z
Plasmid#218909Purposegolden gate cloningDepositorInsertmPGK-Neomycine-SV40 polyA
UseTagsExpressionMammalianMutation395T>CPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-PuroR-SV40 polyA-Z
Plasmid#218908Purposegolden gate cloningDepositorInsertmPGK-PuroR-SV40 polyA
UseTagsExpressionMammalianMutation297T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-BlastR-Z
Plasmid#218907Purposegolden gate cloningDepositorInserthPGK-BlastR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-NeoR-Z
Plasmid#218906Purposegolden gate cloningDepositorInserthPGK-NeoR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-HygroR-Z
Plasmid#218905Purposegolden gate cloningDepositorInserthPGK-HygroR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-BlastR-SV40 polyA-Z
Plasmid#218903Purposegolden gate cloningDepositorInsertmPGK-BlastR-SV40 polyA
UseTagsExpressionMammalianMutation2468T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
(R)pGPD1(D)_P1(SBE109)
Plasmid#194993Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)DepositorInsert(R) pGPD (D)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
(F)pGPD(G)_P2 (SBE110)
Plasmid#194992Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)DepositorInsert(F) pGPD (G)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
(I) pADH2 (J) P3 (SBE114)
Plasmid#187629Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)DepositorInsert(I) pADH2 (J)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F) pADH2 (G)_P2 (SBE113)
Plasmid#187628Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)DepositorInsert(F) pADH2 (G)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)
Plasmid#195054Purposeintegration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterizationDepositorInsertNAT
UseTagsExpressionBacterialMutationPromoterpLDP1Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)
Plasmid#195053Purposeintegration cassette for ku70 deletion and expression of NAT gene under pFBA for characterizationDepositorInsertNAT
UseTagsExpressionBacterialMutationPromoterpFBAAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)
Plasmid#195052Purposeintegration cassette for ku70 deletion and expression of NAT gene under pXYL for characterizationDepositorInsertNAT
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)
Plasmid#195051Purposeintegration cassette for ku70 deletion and expression of NAT gene under pGPD for characterizationDepositorInsertNAT
UseTagsExpressionBacterialMutationPromoterpGPDAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)
Plasmid#195050Purposeintegration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterizationDepositorInsertNAT
UseTagsExpressionBacterialMutationPromoterpADH2Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only