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Plasmid#118391Purposestable lentiviral expression of cDNADepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-FAM134B
Plasmid#109026Purposestable lentiviral expression of cDNADepositorInsertFAM134B (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-ChETA-EYFP
Plasmid#26967Purpose3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorInserthChR2(E123T/H134R)-EYFP
UseLentiviralTagsEYFPExpressionMammalianMutationE123TPromoterCaMKIIaAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pALM179
Plasmid#82023Purposean updated version of cLac143 (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expressionDepositorInsertYFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shFAM134B
Plasmid#109013Purposeshort-hairpin knockdown of targeted geneDepositorInsertshFAM134B (RETREG1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
rLAMP1-mCherry-EGFP-Tet-On
Plasmid#109015PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)DepositorInsertrLAMP1
UseLentiviralTagsmcherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/VChR1-EYFP
Plasmid#20955PurposeMammalian expression of VChR1 fused to EYFP, for neuronal excitationDepositorInsertvolvox channelrhodopsin-1
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGCG26
Plasmid#82371PurposeYFP expressed from pJ23119 in the glpK locus with gentamicin resistanceDepositorInsertYFP
UseTagsExpressionMutationPromoterPJ23119Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE
Plasmid#20294PurposeLentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to SerinePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
p005kanRFP
Plasmid#58533PurposeRFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertRed fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB3C5-proD-B0032-E0051
Plasmid#107241PurposeExpresses lacZa-GFP fusion from constitutive proD promoterDepositorInsertProA-RBS(B0032)-Gemini (E0051)
UseTagsExpressionMutationPromoterproDAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Venus Cam KII alpha mutant T286D
Plasmid#29429DepositorInsertVenus CAM KII Alpha
UseTagsVenusExpressionMammalianMutationT286D mutation in CamKIIa sequencePromoterAvailable sinceJuly 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE
Plasmid#20293PurposeLentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to AlaninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
Venus Cam KII alpha mutant T286A
Plasmid#29430DepositorInsertVenus CAM KII Alpha
UseTagsVenusExpressionMammalianMutationT286A mutation in CamKIIa sequencePromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE
Plasmid#20295PurposeLentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to ThreoninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSB3C5-proA-B0032-E0051
Plasmid#107244PurposeExpresses lacZa-GFP fusion from constitutive proA promoterDepositorInsertProA-RBS(B0032)-Gemini (E0051)
UseTagsExpressionMutationPromoterproAAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB3C5-proC-B0032-E0051
Plasmid#107242PurposeExpresses lacZa-GFP fusion from constitutive proC promoterDepositorInsertProC-RBS(B0032)-Gemini (E0051)
UseTagsExpressionMutationPromoterproCAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB3C5-proB-B0032-E0051
Plasmid#107243PurposeExpresses lacZa-GFP fusion from constitutive proB promoterDepositorInsertProB-RBS(B0032)-Gemini (E0051)
UseTagsExpressionMutationPromoterproBAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALM174
Plasmid#82022Purposean updated version of cLac94 (pACSA_PcptOO_YFP_PMB2__LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expressionDepositorInsertYFP
UseTagsExpressionMutationPromoterPcptooAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas61
Plasmid#82397PurposeIPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpKDepositorInsertLDH
UseTagsExpressionMutationV39RPromotercLac143Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only