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Plasmid#126711Purposeyeast plasmid complementing ALD1 from Komagataella phaffiiDepositorInsertald1
UseTags6xHis,MycExpressionYeastMutationPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ADH3
Plasmid#126710Purposeyeast plasmid complementing ADH3 from Komagataella phaffiiDepositorInsertadh3
UseTags6xHis,MycExpressionYeastMutationPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_lacO-cP-AOX1-sAR
Plasmid#126743Purposeyeast plasmid overexpressing CPR and sLovADepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrps3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Peft2-CAD-Tklura3
Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPeft2Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsmtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Psptdh3-ACO-Tagtef1
Plasmid#127734PurposeYeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsptdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppgk1-PYC-Ttpi1
Plasmid#127735PurposeYeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Synthetic, Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpgk1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppxr1-PYC-Tnat1
Plasmid#127736PurposeYeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Synthetic, Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpxr1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Psbtdh3-CSC-Tefm1
Plasmid#127742PurposeYeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsbtdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Peno2-ACDH-Ttal1
Plasmid#127743PurposeYeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPeno2Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Pvma6-ACDH-Trps14a
Plasmid#127744PurposeYeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPvma6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Prpl28-ACDH-Tecm10
Plasmid#127745PurposeYeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrpl28Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Ptdh3-ACDH-Trpl3
Plasmid#127746PurposeYeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPtdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
CYN2/150 (E. coli Entner-Doudoroff v11)
Bacterial Strain#71420PurposeThis E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway with an improved NADPH regeneration rate. Module is Cm resistantDepositorBacterial ResistanceChloramphenicolAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
CYN1 (E. coli Entner-Doudoroff v1)
Bacterial Strain#71421PurposeThis E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway (first version). The module also contains a Cm resistance marker.DepositorBacterial ResistanceChloramphenicolAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1_IVT
Plasmid#222342PurposePlasmid for evolved and engineered Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTemplate for in vitro transcriptionTagsExpressionMutationPromoterT7 (inactivated)Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA1 with trimmed attP
Plasmid#222346PurposepegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA2 with trimmed attP
Plasmid#222347PurposepegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-Nme2ABE8e
Plasmid#189928PurposePlasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertNme2ABE8e
UseTagsNLSExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only