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We narrowed to 51,996 results for: cloning

Showing: 861 - 880 of 51996 results

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C453-E14: attB4-EF1p>-attB5

Plasmid

#162975

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C455-E01: attB4-stuffer-attB5

Plasmid

#162983

Purpose

Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C515-E01: attB5r-stuffer-attB1r

Plasmid

#162968

Purpose

Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C511-E02: attB5r-NanoLuc-nostop-attB1r

Plasmid

#162938

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E33: attB4-UbCp>-attB1r

Plasmid

#162925

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human Ubiquitin C promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ubiquitin C promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E08: attB2r-3xFLAG-attB3

Plasmid

#162912

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E29: attB2r-mClover3-attB3

Plasmid

#162906

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mClover3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E30: attB2r-mRuby3-attB3

Plasmid

#162907

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mRuby3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E25: attB2r-SNAP-attB3

Plasmid

#162914

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal SNAP tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E02: attB4-CAGp>-attB5

Plasmid

#162972

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the CAG promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

chicken beta-actin/CMV enhancer promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E24: attB4-UbCp>-attB5

Plasmid

#162980

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human Ubiquitin C promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ubiquitin C promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E23: attB5r-mRuby3-nostop-attB1r

Plasmid

#162945

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E22: attB5r-mClover3-nostop-attB1r

Plasmid

#162944

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C238-E02: attB2r-BGHpolyA-attB3

Plasmid

#162916

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Bovine growth hormone polyadenylation sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E23: attB4-TRE3Gp>-attB5

Plasmid

#162979

Purpose

Gateway attB4/attB5 entry clone for inducible mammalian expression using the TRE3G promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Tet-regulated TRE3G minimal promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E04: attB5r-mCherry-nostop-attB1r

Plasmid

#162941

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E31: attB4-TRE3Gp>-attB1r

Plasmid

#162924

Purpose

Gateway attB4/attB1r entry clone for inducible mammalian expression using the TRE3G promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Tet-regulated TRE3G minimal promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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p4P1R_EC1.2en-EC1.1p

Plasmid

#213912

Purpose

Entry clone containing Egg cell-specific promoter (EC1.1p) fused to EC1.2 enhancer (EC1.2en). For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Ziqiang P. et al Bio Protocol, Vol 14, Iss 13, Jul 5, 202

Insert

EC1.2en-EC1.1p

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K

Plasmid

#214460

Purpose

Gateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CSNK1G3 (CSNK1G3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K

Plasmid

#214458

Purpose

Gateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CSNK1G1 (CSNK1G1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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