Addgene

We narrowed to 3,954 results for: chm

Showing: 861 - 880 of 3954 results

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pCellFree_G03 BATF3

Plasmid

#67050

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BATF3 (BATF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry

Plasmid

#133322

Purpose

Bacterial expression of TDP43 with FYW-S mutant CTD

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Bacterial

Mutation

All FYW in the TDP43 IDRs mutated to S

Promoter

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSR357.9

Plasmid

#125105

Purpose

Expression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)129
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1413 GFP-TDP43 VLIM-F

Plasmid

#118802

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

All VLIM to F in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1411 GFP-TDP43 FYW-S

Plasmid

#118800

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

All FYW to S in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pKD279

Plasmid

#125099

Purpose

Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4388(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 EBNA1BP2

Plasmid

#67072

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GTF2E1

Plasmid

#67089

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2E1 (GTF2E1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pHBS1415 GFP-TDP43 VLIM-S

Plasmid

#133323

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

ALL VLIM in the TDP43 IDRs mutated to S

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

Plasmid

#107858

Purpose

To knock-out TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

gRNA against TARDBP (Exon 2) (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 FOXM

Plasmid

#67118

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

FOXM (FOXM1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pSpCAS9n(BB)-2A-puro DNM1, A

Plasmid

#107795

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCAS9n(BB)-2A-puro DNM1, B

Plasmid

#107796

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _701-902

Plasmid

#86821

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1412 GFP-TDP43 FYW-L

Plasmid

#118801

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

All FYW to L in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET21 Catalytic Domain of MALT1

Plasmid

#48970

Purpose

Expresses catalytic domain of MALT1 in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-Scara3-IRES-BFP

Plasmid

#117265

Purpose

Retroviral overexpression of Scara3

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Scara3 (Scara3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

LTR

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

Plasmid

#118279

Purpose

Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

Depositor

Dietmar Schmitz

Article

Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

Inserts

mRuby2
ChR2(H134R)

Use

AAV and Cre/Lox

Tags

SV40 NLS and YFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _462-699

Plasmid

#86819

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1(-) + SLNCR1 _568-637

Plasmid

#86820

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge