-
Plasmid#210295PurposeActuAtor for FKBP-FRB CIDDepositorInsertmActA-FKBP-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AS1371D
Plasmid#224417PurposeMammalian expression of KIF 13a S1371DDepositorInsertKIF 13a S1371D (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationS1371DPromoterChicken Beta actinAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
px552-sg-gria1-HT
Plasmid#187652PurposeContains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.DepositorInsertsHaloTag donor sequence
miRFP670
UseAAV and CRISPRTagsN/AExpressionMammalianMutationPromoterSynapsinAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AS1371A
Plasmid#224409PurposeMammalian expression of KIF 13a S1371ADepositorInsertKIF 13a S1371A (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationS1371APromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AWLDLE
Plasmid#224410PurposeMammalian expression of KIF 13a WLDLEDepositorInsertKIF 13a WLDLE (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationWLDLEPromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1015A-E1021A
Plasmid#213417PurposeVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnTS-E1015A-E1021A (VCL Chicken, Synthetic)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to aā¦PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VcnTS-E1015A-E1021A
Plasmid#213411PurposeVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.DepositorInsertVcnTS-E1015A-E1021A (VCL Chicken, Synthetic)
UseLentiviralTagsExpressionMutationMutated vinculin glutamic acid 1015 and 1021 to aā¦PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1015A-E1021A
Plasmid#213414PurposeVinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnCS-E1015A-E1021A (VCL Chicken, Synthetic)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to aā¦PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Plekhh1 5 silent mutations
Plasmid#187373PurposeExpresses human Plekhh1 with 5 silent mutations labelled with CherryDepositorInsertPlekhh1 with 5 silent mutations
UseTagsmCherryExpressionMammalianMutationFive silent mutations at the Plekhh1 siRNA site (ā¦PromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-EGFP
Plasmid#174477PurposeExpress EGFP with actin-targeting sequence in mammalian cellsDepositorInsertEGFP with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cereviā¦ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-SECFP
Plasmid#174476PurposeExpress SECFP with actin-targeting sequence in mammalian cellsDepositorInsertSECFP with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cereviā¦ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-Sirius
Plasmid#174475PurposeExpress Sirius with actin-targeting sequence in mammalian cellsDepositorInsertSirius with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cereviā¦ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-mfn2 guide
Plasmid#121993PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-mfn2 guide#2
Plasmid#121994PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-opa1 guide
Plasmid#121995PurposeAn entry vector with U6a and U6c promoter driving opa1 guide RNAs expressionDepositorInsertopa1 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_001
Plasmid#123782PurposeEncodes human beta actin as a Type 3b part to be used in the MTK systemDepositorInsertbeta actin (human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-LSS-SGFP2-N1
Plasmid#112938PurposeVisualization of F-actinDepositorInsertLifeAct
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLN-deltaABD-mEos3.2
Plasmid#126812Purposeexpress filamin with actin binding domain deletedDepositorInsertfilamin deltaABD-mEos3.2
UseDictyostelium expressionTagsmEos3.2ExpressionMutationPromoterAvailable sinceAug. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mScarletI pDRF1-GW
Plasmid#168085PurposeExpresses Lifeact-mScarletI to visualize actin in yeastDepositorInsertLifeAct-mScarletI
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mTq2 pDRF1-GW
Plasmid#168084PurposeExpresses Lifeact-mTq2 to visualize actin in yeastDepositorInsertLifeAct-mTq2
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only