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Showing: 861 - 880 of 3847 results

  1. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

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  2. IBMc226

    Plasmid
    #161944
    Purpose
    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161944-plasmid-map-sequence-id-326341

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  4. pYSD313

    Plasmid
    #212931
    Purpose
    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    Sed1 (SED1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212931-plasmid-map-sequence-id-421768

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  6. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid
    #174660
    Purpose
    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.
    Depositor
    Beverly L. Davidson
    Article
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Insert
    miniXon cassette
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174660-plasmid-map-sequence-id-351622

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  7. pGL4.10/RSV_SF3B3-Xon_Luciferase

    Plasmid
    #174659
    Purpose
    Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.
    Depositor
    Beverly L. Davidson
    Article
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Insert
    Xon cassette
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174659-plasmid-map-sequence-id-351621

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  8. CAG sHRPa-FRB-pre mGRASP

    Plasmid
    #73145
    Purpose
    Large fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-FRB-pre mGRASP
    Use
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73145-plasmid-map-sequence-id-134517

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  9. Synapsin sHRPa-NRX in FSW lentiviral vector

    Plasmid
    #73147
    Purpose
    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-NRX
    Use
    Lentiviral
    Tags
    2x HA tag and Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73147-plasmid-map-sequence-id-134524

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  15. FKBP-CD28TMD-NTEVp(H75E)

    Plasmid
    #137829
    Purpose
    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease
    Depositor
    Joshua Leonard
    Article
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Insert
    FKBP-CD28TMD-NTEVp(H75E)
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Histidine 75 to Glutamic Acid in NTEVp fr…
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137829-plasmid-map-sequence-id-311953

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  17. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid
    #137830
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Article
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Insert
    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Leucine 71 to Lysine in CTEVp fragment
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137830-plasmid-map-sequence-id-311956

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  20. dCas9-NLS-gs10-cTRb413

    Plasmid
    #188261
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cTRb413 (TXNRD2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188261-plasmid-map-sequence-id-374572

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Showing: 861 - 880 of 3847 results