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Showing: 8761 - 8780 of 86880 results

  1. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Article
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and hPGK
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  12. pCP60 RS-GFP

    Plasmid
    #122173
    Purpose
    "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"
    Depositor
    Lukas Fischer
    Article
    Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1
    Insert
    RS-GFP
    Use
    Binary vector for escherichia coli and agrobacter…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122173-plasmid-map-sequence-id-238572

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  15. pUDP004

    Plasmid
    #101165
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101165-plasmid-map-sequence-id-199321

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  19. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110095-plasmid-map-sequence-id-317064

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Showing: 8761 - 8780 of 86880 results