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Plasmid#53350Purposebacterial expression of mouse PRDM16 fragmentDepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsGSTExpressionBacterialMutationaa 880-1038PromotertacAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
GST-PRDM16 224-454
Plasmid#53347Purposebacterial expression of mouse PRDM16 fragmentDepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsGSTExpressionBacterialMutationaa 224-454PromotertacAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SSpB-HaloTag-p63DH
Plasmid#176116PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper-T7IS1
Plasmid#140631PurposeExpresses ShCAST under the T7 promoter and an empty sgRNADepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-Actin-C-18
Plasmid#158009PurposeVisualization of actin in mammalian cells using mGoldDepositorInsertActin (ACTB Human)
UseTagsmGoldExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
1129 pcDNA3 GFP FKHR H215R AAA
Plasmid#9034DepositorInsertFKHR H215R AAA (FOXO1 Human)
UseTagsGFPExpressionMammalianMutationH215R T24A S256A S319APromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
GST-PRDM16 1-223
Plasmid#53346Purposebacterial expression of mouse PRDM16 fragmentDepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsGSTExpressionBacterialMutationaa 1-223PromotertacAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-Keratin-N-17
Plasmid#158001PurposeVisualization of keratin using mGoldDepositorInsertmGold-Keratin-N-17 (KRT18 Human)
UseTagsmGoldExpressionMammalianMutationV282LPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SlugABEmax
Plasmid#163798PurposeExpresses ABEmax and nSlugCas9DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationSlugCas9 (D10A)PromoterAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-cr in PBCSK-
Plasmid#126641PurposeCloning vector LbCas12a DR-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-PRDM16 1039-1176
Plasmid#53351Purposebacterial expression of mouse PRDM16 fragmentDepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsGSTExpressionBacterialMutationaa 1039-1176PromotertacAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
GST-PRDM16 681-880
Plasmid#53349Purposebacterial expression of mouse PRDM16 fragmentDepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsGSTExpressionBacterialMutationaa 681-880PromotertacAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
GST-PRDM16 455-680
Plasmid#53348Purposebacterial expression of mouse PRDM16 fragmentDepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsGSTExpressionBacterialMutationaa 455-680PromotertacAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMGIB-HA-hTCAB1
Plasmid#167460PurposeRetroviral expression of HA-TCAB1 (WDR79) in mammalian cellsDepositorInsertTCAB1 (WRAP53 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
YL-CashGem-BAT1-URA3-LINEAR
Plasmid#174837PurposepRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolyticaDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterGPDpAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-RFP
Plasmid#65448PurposeExpression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectorsDepositorInsertSV40-RFP
UseLentiviralTagsExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Poly(A)‐NeoR
Plasmid#65526PurposeNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-p97-EQ-mycStrep
Plasmid#31838DepositorInsertp97
UseTagsStrep and mycExpressionBacterial and MammalianMutationE578QPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-WT
Plasmid#64061Purposemyc-tagged murine seipin (wild type); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
promoter J23110 (upstream of RBS, mRFP1)
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only