Addgene

We narrowed to 37,879 results for: ina

Showing: 8721 - 8740 of 37879 results

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Cntn1-Fc-His

Plasmid

#72065

Purpose

Expresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn1 (Cntn1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/38R-1]

Plasmid

#206719

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/38-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant Mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-GST [N100/13R ]

Plasmid

#188214

Purpose

Mammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLM-AMA18.0 dCas9-VPR

Plasmid

#138945

Purpose

Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers

Depositor

Arnold Driessen

Article

Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

Insert

p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers

Use

CRISPR and Synthetic Biology; Ama1 autonomously r…

Tags

NLS

Expression

Mutation

Promoter

p40S (AN0465) Aspergillus nidulans

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Use

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-EDA in pMAX

Plasmid

#120402

Purpose

enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Article

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Insert

EDA-fibronectin (FN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-QKI [N147/6.1R]

Plasmid

#114521

Purpose

Mammalian Expression Plasmid of anti-Pan-QKI (Human). Derived from hybridoma N147/6.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-QKI (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.AEBP2

Plasmid

#125165

Purpose

Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Zinc finger protein AEBP2 (AEBP2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2

Plasmid

#59904

Purpose

Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2

Plasmid

#125161

Purpose

Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EZH2 (EZH2 Human)

Use

Baculovirus expression vector

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EED

Plasmid

#125163

Purpose

Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eC

Plasmid

#201903

Purpose

non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

Insert

Synthetic construct isolate AAV-PHP.eC VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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t1-405

Plasmid

#166127

Purpose

For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

Depositor

Vesa Hytönen

Article

Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

Insert

Tln1Head (1-405) (TLN1 Human)

Use

Tags

His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

Expression

Bacterial

Mutation

residues 1-405 only

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/8R]

Plasmid

#177572

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EF-FH-TAZ S89A-ires-blast

Plasmid

#52084

Purpose

Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

Depositor

Yutaka Hata

Article

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

TAZ S89A (WWTR1 Human)

Use

Lentiviral

Tags

FLAG and His

Expression

Mammalian

Mutation

S89A

Promoter

EF1a

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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Jam2-Fc-His

Plasmid

#72079

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)

Plasmid

#65446

Purpose

Expression of ephrinB1 and RFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

ephrin B1 (Efnb1 Mouse)

Use

Lentiviral

Tags

SV40-RFP

Expression

Mammalian

Mutation

Promoter

UBQ

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGEX4T2-Ste7-EE

Plasmid

#52681

Purpose

expression of constitutively active Ste7 kinase in bacteria

Depositor

Benjamin Turk

Article

Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

Insert

Ste7 (STE7 Budding Yeast)

Use

Tags

GST and Myc

Expression

Bacterial

Mutation

S359E/T363E constitutively active

Promoter

tac

Available since

April 30, 2014

Availability

Academic Institutions and Nonprofits only