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Plasmid#122088Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only
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pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination
Plasmid#40131PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).DepositorTypeEmpty backboneUseTagsFokI nuclease (KKR)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
Golgi-targeted optoPLD
Plasmid#140058PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expressionDepositorInsertPhospholipase D
UseTagsCIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-Rsn2
Plasmid#61686Purposebacterial expression of ranaspumin 2 as a His-tagged fusion proteinDepositorInsertRanaspumin-2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
UseTagsGSGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.21_mCherry-MS2x6
Plasmid#205536PurposeExpress mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330S-5
Plasmid#58781PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-pBAD
Plasmid#54838PurposeLocalization: Protein Expression Vector , Excitation: 530, Emission: 541DepositorTypeEmpty backboneUseTags6xHis and mPapayaExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCarmine in pRSETB
Plasmid#109484Purposebacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)DepositorInsertmCarmine
UseTagsN-terminal histidine tag in vectorExpressionBacterialMutationPromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-HIS
Plasmid#160142PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI4-MTU-DO-HPH
Plasmid#160216PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-URA
Plasmid#160015PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-LEU
Plasmid#160013PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKSB-609 U6-sgRNA1 ATCC GGAA
Plasmid#173671PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-BCCP-PupE
Plasmid#113403PurposeExpresses BCCP-PupE in bacteriaDepositorInsertBCCP-PupE
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0000
Plasmid#90997PurposeModule A, Promoter: none, Gene: none, Terminator: noneDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAC-BA-lacZ
Plasmid#13423DepositorInsertBA
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTD-CTwinStrep
Plasmid#45939Purposeuse to create C-terminal Twin-Strep-tag fusion proteinsDepositorTypeEmpty backboneUseTagsTwin-Strep-tagExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKSB-610 U6-sgRNA2 GGAA AGAG
Plasmid#173672PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-MYC-e5
Plasmid#120343PurposepGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.DepositorInsertchr8:130594100-130596043 (hg19)
UseLuciferaseTagsExpressionMammalianMutationPromoterMYCAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only