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Showing: 8721 - 8740 of 22333 results

  1. pRS415-Cas9-VPR

    Plasmid
    #163971
    Purpose
    Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast
    Depositor
    Jiazhang Lian
    Article
    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22
    Insert
    Cas9-VPR
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    163971-plasmid-map-sequence-id-323600

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  7. pCIFR-8

    Plasmid
    #227666
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Am) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    AmR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227666-plasmid-map-sequence-id-458899

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  8. pFNC-8

    Plasmid
    #227670
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. AmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    AmR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227670-plasmid-map-sequence-id-458895

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  9. pAM59

    Plasmid
    #227655
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, Δ78–81
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227655-plasmid-map-sequence-id-455288

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  10. LPUtopia-8

    Plasmid
    #224781
    Purpose
    Landing Pad donor vector for AAVS1 site integration in human and VeroE6 cells
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Inserts
    puromycin N-acetyltransferase
    enhanced Green Fluorescence Protein
    Red Fluorescence Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224781-plasmid-map-sequence-id-445949

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  11. GB1531

    Plasmid
    #215242
    Purpose
    TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized.
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    PNos:PhiC31:TNos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215242-plasmid-map-sequence-id-436558

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  12. pGWB511-TP-BVRA

    Plasmid
    #203480
    Purpose
    For Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter.
    Depositor
    Yutaka Kodama
    Article
    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:
    Insert
    Rattus rattus biliverdin reductase A flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203480-plasmid-map-sequence-id-404963

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  13. pILGFP4D41

    Plasmid
    #185841
    Purpose
    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185841-plasmid-map-sequence-id-369628

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  15. pILGFP8EFA

    Plasmid
    #185854
    Purpose
    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    DHFR P66L
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185854-plasmid-map-sequence-id-370766

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Showing: 8721 - 8740 of 22333 results