Addgene

We narrowed to 16,572 results for: grna

Showing: 8661 - 8680 of 16572 results

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pWS5393-CRISPRai-TRP1

Plasmid

#182832

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - TRP1 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5395-CRISPRai-LYS2

Plasmid

#182834

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - LYS2 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5399-CRISPRai-ZeoR

Plasmid

#182838

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5391-CRISPRai-LEU2

Plasmid

#182830

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - LEU2 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1m_v1

Plasmid

#97083

Purpose

Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1m_v1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMM136

Plasmid

#127217

Purpose

BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, MP-delta, sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pX335-NEAT1_IS_v1

Plasmid

#97085

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pOC2 Tubb3-GFP KI

Plasmid

#183432

Purpose

CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-NEAT1_IS_v2

Plasmid

#97086

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v2

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV/MASC_(GLuc)_TOP1

Plasmid

#68439

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pOC1 Gria1-GFP-FRB KI

Plasmid

#183428

Purpose

CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP-FRB donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Dlg4-Halo-FKBP KI

Plasmid

#183435

Purpose

CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo-FKBP donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pC1.530

Plasmid

#205441

Purpose

Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

RepB gRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFH6_new

Plasmid

#105866

Purpose

more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

Depositor

Andreas Weber

Article

Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

Insert

U6-26p::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Subcloning

Tags

Expression

Plant

Mutation

Promoter

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB6638

Plasmid

#106165

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN12RRFuzzy3MYB

Plasmid

#194441

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA array targeting CEN1, RR, Fuzzy3MYB

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

MtU6

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pOC3 smFP-HA-Cacna1e KI

Plasmid

#183436

Purpose

CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-GFP-SFPQ

Plasmid

#97084

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA GFP-SFPQ

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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