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Plasmid#168971PurposeGateway-compatible Entry vectorDepositorInsertHCoV-229E-Spike (S )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDipA-TEV-His12
Plasmid#137042Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP1356
Plasmid#119276Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE
Plasmid#139088PurposeExpresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.DepositorInsertxCas9 3.7
UseCRISPR and LentiviralTagsExpressionMammalianMutationA262T, R324L,S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA_CDKN1A
Plasmid#136538PurposeLentiviral expression vector for an inducible BirA-CDKN1ADepositorInsertBirA-CDKN1A (CDKN1A )
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
Plasmid#172514PurposeEncodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoterDepositorInsertpAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV Renilla Luciferase L5-L2
Plasmid#62186PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloningDepositorInsertRenilla luciferase
UseLuciferase; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_linker
Plasmid#211731PurposeLinker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regionsDepositorInsertLinker fragment SARS-CoV-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK-miR-30 L1-L4
Plasmid#62180PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1-miR-30 L1-R5
Plasmid#62181PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-2-nsp16
Plasmid#168876PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-2-nsp16 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3CY56
Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-shRNA
Plasmid#82697PurposeFor targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.DepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterhPGK, U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP1237
Plasmid#119262Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMA7-SacB
Plasmid#79968PurposeTM-MAGE strainDepositorInsertsLambda Red recombinase beta subunit
DNA adenine methylase
Levansucrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
p221z-erVenusYFP
Plasmid#71265PurposeEntry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertVenusYFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-OsU6a/LacZ
Plasmid#66195Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterOsU6aAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-2-Nucleocapsid
Plasmid#168886PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-2-Nucleocapsid (N )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-B2705-KK10-SABR
Plasmid#119053Purpose3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBIDepositorInsertB2705-KK10-SABR
UseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-4eCOL2A1
Plasmid#97211PurposeFor cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectorsDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseVector is designed for cloning and generation of …TagsExpressionMutationPromoterCOL2A1 regulatory elementsAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only