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pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

Plasmid

#69233

Purpose

R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Promoter

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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mKO-Alpha-Actinin-19

Plasmid

#57831

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mKO

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP eIF2beta C-term (aa 89-331)

Plasmid

#45901

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2S2 aa89-331 (Eif2s2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 89-331

Promoter

CMV

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pRSETC-PITPbeta (splice variant 1) K61A

Plasmid

#58271

Purpose

bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL* EPSM

Plasmid

#27005

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-203; T179V

Promoter

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL* WT

Plasmid

#27042

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-203

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pQCXIH-Flag Smad3 AAGY

Plasmid

#27027

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

P181A P182A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL AAGY

Plasmid

#27041

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226; P181A P182A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM

Plasmid

#27222

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A

Promoter

Available since

Dec. 21, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 AAGY

Plasmid

#27013

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

P181A P182A

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S65D

Plasmid

#13621

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Ser 65 to Asp

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT T69D

Plasmid

#13622

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Thr 69 to Asp

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S70D

Plasmid

#13623

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Ser 70 to Asp

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S65A

Plasmid

#13618

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

PARL (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65 to Alanine

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT T69A

Plasmid

#13619

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Thr 69 to Ala

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pAV10

Plasmid

#63213

Purpose

pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pET41a iGlum

Plasmid

#119829

Purpose

Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGlum

Use

Tags

GST

Expression

Bacterial

Mutation

R24K

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1 pET24b

Plasmid

#128088

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

Depositor

Dan Minor

Article

Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

Insert

NaVSp1p

Use

Tags

3C Cleavage, His Tag, and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pET28a-PHGDH

Plasmid

#74113

Purpose

Expression of N-terminal His-tagged

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

PHGDH (PHGDH Human)

Use

Tags

His-tagged

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc FL

Plasmid

#221853

Purpose

Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

calreticulin (CALR Human)

Use

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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