Addgene

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pGEX-HA-GlpG

Plasmid

#155135

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

tac

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt7B-V5

Plasmid

#35934

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS2A

Plasmid

#145353

Purpose

Cell-free expression vector for Zika virus NS2A protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Use

Tags

mCherry and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R1R2-pNeo

Plasmid

#141057

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM55361

Plasmid

#153227

Purpose

Control plasmid for knockout of AP3 gene

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Cas9, guide RNA for TRY and CPC and FAST marker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-SGTAP no-pA

Plasmid

#82599

Purpose

3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

SBP-TEV-2x protein G

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt9A-V5

Plasmid

#35937

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation A (543) --> G

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFLIPglu-3.2mDelta13

Plasmid

#12992

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A S112A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

F16A, S112A in binding protein

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pME-QF2w-SV40pA

Plasmid

#155117

Purpose

Middle entry vector containing QF2w

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QF2w

Use

Gateway middle entry vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFLIPglu-170nDelta13

Plasmid

#12989

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16F Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

WT binding protein (no F16A)

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt6-V5

Plasmid

#35932

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (567) --> A

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-16

Plasmid

#160716

Purpose

T-nos Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

nopaline synthase terminator (T-nos) bordered by BsaI restriction sites

Use

Synthetic Biology

Tags

Expression

Mutation

Incorporation of BsaI restriction sites

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-13

Plasmid

#160713

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA) bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV PrM

Plasmid

#145350

Purpose

Cell-free expression vector for Zika virus prM protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Use

Tags

mCherry and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFU-iMVIIA-PE

Plasmid

#24146

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP-IRES-TetR-KRAB (PDGFRB Conus magus, Aequorea victoria, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1

Plasmid

#141025

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-Neo-L2

Plasmid

#141007

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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vector plix 4.03

Plasmid

#121130

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt8B

Plasmid

#35917

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8B (WNT8B Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_EV3

Plasmid

#102293

Purpose

Yeast Surface Display of 5Ins_16_EV3

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Evolved Insulin binding scFV

Use

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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