Showing: 8641 - 8660 of 25499 results
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Plasmid#192447PurposeNon-phosphorylatable negative-control mutant of ExRai-AMPKAR.DepositorInsertExRai-AMPKAR(T/A)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationThr 6 in AMPK substrate mutated to Ala.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-ExRai-AMPKAR
Plasmid#192448PurposeExRai-AMPKAR targeted to the mitochondrial outer membrane.DepositorInsertMito-ExRai-AMPKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AMPKAR
Plasmid#192450PurposeExRai-AMPKAR targeted to the nucleus.DepositorInsertExRai-AMPKAR-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28b_RanT24N
Plasmid#186271PurposeExpression vector of His-RanT24N in E.coliDepositorInsertGTP-binding nuclear protein Ran
UseTagsHis tagged and His-tagged at N terminalExpressionBacterialMutationChanged T24 into NPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pETM33_PDCD6IP_ALIX V-domain
Plasmid#178483PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertPDCD6IP_ALIX V-domain (PDCD6IP Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailabilityAcademic Institutions and Nonprofits only -
pETM33_PTK2_Focal-AT
Plasmid#178485PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertPTK2_Focal-AT (PTK2 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailabilityAcademic Institutions and Nonprofits only -
pETM33_ZYMND11_coiled-coil MYND
Plasmid#178493PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailabilityAcademic Institutions and Nonprofits only -
Lyn11-BirA*-HA
Plasmid#214474PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.DepositorInsertBirA(R118G)
UseLentiviral; GatewayTagsHA tag and Lyn11 (Plasma Membrane Localization Se…ExpressionMammalianMutationR118GPromoterEF-1aAvailabilityAcademic Institutions and Nonprofits only -
pSD118A
Plasmid#218140PurposeExpresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coliDepositorInsertSD0_ZF2-3
UseTagsAviTag-6xHis-MBP-TEV-(GGGGS)2ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R/K63R)-yeGFP-His6
Plasmid#212785PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMA3200
Plasmid#46855PurposeA lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12DepositorInsertExoY
UseLentiviralTagsFKBP E31G, R71G, K105E and MycExpressionMutationPromoterTRE-TightAvailabilityAcademic Institutions and Nonprofits only -
pMA3228
Plasmid#46856PurposeA lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12DepositorInsertExoY K81M
UseLentiviralTagsFKBP E31G, R71G, K105E and MycExpressionMutationPromoterTRE-TightAvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Mst2
Plasmid#84296PurposeExpresses Myc-tagged Mst2DepositorInsertMst2 (STK3 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SpCas9
Plasmid#104588PurposeAAV vector expressing Myc-tagged SpCas9 under the EFS promoterDepositorInsertStreptococcus pyogenes Cas9
UseAAV and CRISPRTagsMycExpressionMammalianMutationPromoterEFSAvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorUseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-SMO-1(GG)
Plasmid#87054PurposeExpresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORFDepositorInsertsmo-1 (smo-1 Nematode)
UseTags6xHis-TEVExpressionBacterialMutationThe last aa was removed to render processed SMO-1…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-173-N-ORAI1
Plasmid#87618PurposeN-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.DepositorInsertVenus173-N-ORAI1 (ORAI1 Human, Synthetic)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pVH003
Plasmid#80397PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experimentsDepositorUseSynthetic BiologyTagsFused by GS linker to LZx domain and Venus has N-…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-Venus-FLARE-Cameleon
Plasmid#123351PurposeYellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertVenus-Venus-FLARE-Cameleon
UseTags6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 8641 - 8660 of 25499 results