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Plasmid#26202DepositorInsertI-kappa-B kinase epsilon K38A (IKBKE Human)
UseTagsFlagExpressionMammalianMutationCatalytic Lysine changed to Alanine (K38A)PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
YFP-S100A10
Plasmid#107200PurposeExpresses YFP-human S100A10 fusion protein for live cell imagingDepositorInsertS100A10 (S100A10 Human)
UseTagsYellow Fluorescent ProteinExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF6 VPS51-V5-6His
Plasmid#175502PurposeV5 tagged VPS51 is expressed in mammalian cellsDepositorInsertVPS51 (VPS51 Human)
UseTagsV5ExpressionMammalianMutationA661V- please see depositor commentsPromoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-OC43-Spike
Plasmid#168951PurposeGateway-compatible Entry vectorDepositorInsertHCoV-OC43-Spike (S )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBLO 62.5
Plasmid#123124PurposeHuman expression vector for planctomyces CasX and sgRNADepositorInsertsCasX protein
CasX sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS
Plasmid#199355PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFA-SUMOstarExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
IDE-2Strep
Plasmid#210355PurposeExpresses IDE fused with 2Strep in mammalian cellsDepositorInsertIDE (IDE Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-229E-Spike
Plasmid#168971PurposeGateway-compatible Entry vectorDepositorInsertHCoV-229E-Spike (S )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2-MACROD1
Plasmid#172587PurposeFor transient expression of C-terminal mRuby2-tagged MACROD1DepositorInsertMACROD1 (MACROD1 Human)
UseTagsmRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2-MACROD1_d77
Plasmid#172588PurposeFor transient expression of C-terminal mRuby2-tagged MACROD1del1-77DepositorInsertMACROD1 (MACROD1 Human)
UseTagsmRuby2ExpressionMammalianMutationdeleted amino acids 1-76PromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXA4 (murine) HA-tag pET
Plasmid#8489DepositorInsertHOXA4 (Hoxa4 Mouse)
UseTagsHA and T7ExpressionBacterialMutationThr-2 changed to Ala-2 to form better ribosomal b…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab6 T27N
Plasmid#46782Purposeexpression of T27N Rab6a in mammalian cellsDepositorInsertRab6 T27N (RAB6A Human)
UseTagsmycExpressionMammalianMutationT27N dominant negativePromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11
Plasmid#193752Purposehuman codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGHDepositorInsertEF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-H3.3-polyA
Plasmid#59780PurposeExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertH3F3B (H3-3B Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-Flag
Plasmid#210349PurposeExpresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPS0382
Plasmid#133232PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-STK3
Plasmid#172990Purposeconstitutive expression of FLAG-tagged STK3DepositorInsert3xFLAG-STK3 (STK3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176854PurposeExpresses membrane bound biotin ligase, BioID2, in neuronsDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , residues 108-200
Plasmid#180628PurposeBacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.DepositorInsertSPASTIN
UseTagsHIS-SUMOExpressionBacterialMutationResidues 108-200PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Vkorc1l1
Plasmid#175148PurposeLentiviral expression of V5-tagged mouse Vkorc1l1DepositorInsertVkorc1l1 (Vkorc1l1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only