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Plasmid#14892PurposeBacterial expression of mKalama1 blue fluorescent proteinDepositorInsertmKalama1
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …PromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136B2.0
Plasmid#167160PurposeGolden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
ykgMO-IGS-sfGFP
Plasmid#207231PurposeCalprotectin sensing promoter with sfGFP reporterDepositorInsertykgMO-IGS
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pS3
Plasmid#50596PurposeBackbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5DepositorInsertsaroE
aroD
aroB
aroG*
ppsA
tktA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb
Plasmid#175534PurposeExpression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag7-myc_PDGFRtmb
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Frb-N-TEV
Plasmid#100095PurposeSNIPer plasmid, N terminal TEV fused to FrbDepositorInsertN-TEV
UseRetroviralTagsFrb and MycExpressionMammalianMutationN terminal domainPromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pN7CAST
Plasmid#190661PurposeExpresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2
Plasmid#88915PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-mCherry-p2A-bReaches-TS
Plasmid#158701PurposeReporter constructDepositorInsertmCherry-p2A-bReaches-TS
UseAAVTagsmCherryExpressionMammalianMutationPromoterTREAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ920
Plasmid#42234DepositorInsertCas9
UseCRISPRTagsGFP, HA epitope, and NLS (nuclear localization si…ExpressionMammalianMutationcodon-optimized synthetic DNA sequencePromoterCMVAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSETa_ mEos4a
Plasmid#51072PurposePhotoconverible Fluorescent Protein_ Fixative ResistantDepositorInsertmEos4a
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAVscCBPIGpluc
Plasmid#35645DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterCBAvailable sinceMay 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
hRIP1 HA GFP wt
Plasmid#41396DepositorInsertRIP1 (RIPK1 Human)
UseTagsGFP and HAExpressionMammalianMutationE96GPromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-oriT
Plasmid#32556DepositorInsertoriT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-myc3-ROC1
Plasmid#20717DepositorInsertRING finger protein 1 (ROC1) (RBX1 Human)
UseTags3xMycExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDD104 (Peft-3::Cre)
Plasmid#47551PurposeCre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germlineDepositorInsertCre Recombinase
UseCre/LoxTagsExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMD18-T-pyrF
Plasmid#174388Purposemodified pMD18-T containing pyrF and its native promoter, AmprDepositorInsertpyrF
UseTemplate for cloning fragments for chromosomal in…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only