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Plasmid#158754PurposeExpression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsert6xHistidine_TagRFP-T_A1aY1
UseTags6x-Histidine tag and TagRFP-TExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-tagRFP-KDEL
Plasmid#114177PurposeGateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ERDepositorInserttagRFP-KDEL
UseGateway entry cloneTagsKDEL retention signal and signal peptideExpressionMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV14_NHP2L1(15.5kD)
Plasmid#73065Purposeexpression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tagDepositorInsertNHP2L1 (15.5kD) (SNU13 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEntry_MERS-CoV-Nucleocapsid
Plasmid#168833PurposeGateway-compatible Entry vectorDepositorInsertMERS-CoV-Nucleocapsid (N )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SlugCas9
Plasmid#163793PurposeExpresses SlugCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2
Plasmid#186361PurposeEntry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.DepositorInsertHuman Ensconsin (MAP7 Human, Synthetic)
UseExpression of a fluorescent microtubule markerTags3xEGFPExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-OC43-nsp12
Plasmid#168949PurposeGateway-compatible Entry vectorDepositorInsertHCoV-OC43-nsp12 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-NL63-nsp12
Plasmid#168927PurposeGateway-compatible Entry vectorDepositorInsertHCoV-NL63-nsp12 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-tRNApromo-sgRNA-espCas91_1
Plasmid#167210PurposePlasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)
Plasmid#90086PurposeThis plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNADepositorInsertCas9
UseCRISPR and LentiviralTagsDestabilized Domain and FlagExpressionMammalianMutationPromoterEFSAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-OC43-nsp1
Plasmid#168931PurposeGateway-compatible Entry vectorDepositorInsertHCoV-OC43-nsp1 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-HKU1-nsp1
Plasmid#168893PurposeGateway-compatible Entry vectorDepositorInsertHCoV-HKU1-nsp1 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-1-nsp1
Plasmid#168841PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-1-nsp1 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-OC43-nsp8
Plasmid#168936PurposeGateway-compatible Entry vectorDepositorInsertHCoV-OC43-nsp8 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-NL63-nsp8
Plasmid#168917PurposeGateway-compatible Entry vectorDepositorInsertHCoV-NL63-nsp8 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP1337
Plasmid#119270Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-HKU1-Nucleocapsid
Plasmid#168906PurposeGateway-compatible Entry vectorDepositorInsertHCoV-HKU1-Nucleocapsid (N )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-1-Nucleocapsid
Plasmid#168852PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-1-Nucleocapsid (N )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9-POLD3
Plasmid#183194PurposeExpression cloneDepositorInsertPOLD3
UseCRISPRTagsCas9WTExpressionMammalianMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-OsU6c
Plasmid#66197Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterOsU6cAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only