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Viral Prep#112677-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only
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pDONR223_ETS1_WT
Plasmid#82118PurposeGateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.DepositorInsertETS1 (ETS1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ETS1_WT
Plasmid#82118PurposeGateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.DepositorInsertETS1 (ETS1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hERalpha
Plasmid#101141PurposeMammalian expression of full-length human estrogen receptor-alphaDepositorInsertestrogen receptor-alpha (ESR1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hERalpha
Plasmid#101141PurposeMammalian expression of full-length human estrogen receptor-alphaDepositorInsertestrogen receptor-alpha (ESR1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Phusion
Plasmid#206210PurposeInducible expression of DNA polymeraseDepositorInsertPhusion DNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3HA-hMIB1
Plasmid#33317DepositorInsertMIB1 (MIB1 Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3HA-hMIB1
Plasmid#33317DepositorInsertMIB1 (MIB1 Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-mi3
Plasmid#159995PurposeExpresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccinationDepositorInsertSpyCatcher003-mi3
UseTagsC-TagExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-mi3
Plasmid#159995PurposeExpresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccinationDepositorInsertSpyCatcher003-mi3
UseTagsC-TagExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-tetoff-mVenus-PICdc42
Plasmid#91876PurposePI(WT): Photo inhibitable (blue light sensitive)DepositorInsertVav2 (VAV2 Human)
UseTagsmVenusExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-tetoff-mVenus-PICdc42
Plasmid#91876PurposePI(WT): Photo inhibitable (blue light sensitive)DepositorInsertVav2 (VAV2 Human)
UseTagsmVenusExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-ChR2-mCherry (AAV8)
Viral Prep#137142-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChR2-mCherry (#137142). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Cre and Flp-dependent expression of channelrhodopsin fused to mCherry for optogenetic activation.DepositorPromoternEFTagsmCherry (Cre and Flp-dependent)Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Neo (657-2)
Plasmid#17447Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, NeoDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Neo (657-2)
Plasmid#17447Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, NeoDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR CD22-Megf10 CAR
Plasmid#113016Purposefor production of lentivirus encoding CD22 CAR-P Megf10DepositorInsertCD22 CAR-P Megf10 intracellular domain
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR CD22-Megf10 CAR
Plasmid#113016Purposefor production of lentivirus encoding CD22 CAR-P Megf10DepositorInsertCD22 CAR-P Megf10 intracellular domain
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC G3BP1-GFP-GFP
Plasmid#119950PurposeLentiviral vector expressing G3BP1-GFP fusion proteinDepositorInsertG3BP1 (G3BP1 Human)
UseLentiviralTagsGFPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC G3BP1-GFP-GFP
Plasmid#119950PurposeLentiviral vector expressing G3BP1-GFP fusion proteinDepositorInsertG3BP1 (G3BP1 Human)
UseLentiviralTagsGFPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH-CAG-AtAFB2.F74A-IRES-puro
Plasmid#216245PurposeExpress AtAFB2.F74A mutant without tag for AID2DepositorInsertAtAFB2.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsExpressionMammalianMutationF74A mutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only