Addgene

We narrowed to 24,707 results for: sta

Showing: 8581 - 8600 of 24707 results

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pNP1 AND gate sfGFP

Plasmid

#107361

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

AND gate + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAG249

Plasmid

#33202

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

WBP4 3'UTR (WBP4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pAc5.1A-Dm-miR-2a-1

Plasmid

#22764

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 2a-1 (mir-2a-1 Fly, primary transcript)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-13a

Plasmid

#22761

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 13a (mir-13a Fly, primary transcript)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pJB128 (MatP-mEos2)

Plasmid

#72653

Purpose

Inducible expression of MatP-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

MatP-mEos2

Use

Tags

mEos2

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP-Ofd1-Myc-A80T

Plasmid

#24579

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pAB042b

Plasmid

#79193

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-MBPoff7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDIRECT_37J

Plasmid

#91153

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAB042d

Plasmid

#79194

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-p38_2_3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup88-HindIII

Plasmid

#87329

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-831-1792

Plasmid

#40961

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1010-1792

Plasmid

#40959

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1009

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pMOD_A1310

Plasmid

#91034

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_H840A nickase

Use

CRISPR

Tags

Expression

Mutation

H840A

Promoter

ZmUbi

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-CENPB-N-22

Plasmid

#57360

Purpose

Localization: Nucleus/Centromeres, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CENPB (CENPB Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMOD_A0208

Plasmid

#91004

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Tags

Expression

Mutation

D10A

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEG587

Plasmid

#40196

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)

Use

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

S458A; exon 2 recoded to be RNAi-resistant

Promoter

4.4 kb mex-5 promoter fragment

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pENTR221-WNT3A

Plasmid

#79501

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3A (WNT3A Human)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Flag3-pG-cNanog-pgk-hph

Plasmid

#74912

Purpose

Mammalian expression of Flag tagged cNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAB043a

Plasmid

#79222

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-MBP; PlacIq lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ; PlacIq

Available since

April 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-VPS21

Plasmid

#18842

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

VPS21 (VPS21 Budding Yeast)

Use

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only