Addgene

We narrowed to 13,574 results for: pET

Showing: 841 - 860 of 13574 results

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pET21 E2F1

Plasmid

#90313

Purpose

Expresses human E2F1 in bacteria

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 (E2F1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET32a-HD39Q

Plasmid

#11514

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 39 glutamines (HTT Human)

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 39 glutamines, vector m…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pET28a-DDX43

Plasmid

#134574

Purpose

Express human DDX43 protein (WT, full-length) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43 (DDX43 Human)

Use

Tags

His

Expression

Bacterial

Mutation

full-length WT

Promoter

T7

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-irp3 WT

Plasmid

#87106

Purpose

expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

Insert

irp3 WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET21c-eIF4A

Plasmid

#37230

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF1 (TIF1 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-FAM86A

Plasmid

#85117

Purpose

bacterial expression of FAM86A

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EEF2KMT (FAM86A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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GSK3beta/pET29b

Plasmid

#16315

Depositor

Peter Klein

Article

Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

Insert

GSK3beta (gsk3b.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pET32a-hAREG

Plasmid

#199229

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Amphiregulin preproprotein (AREG Human)

Use

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pET11a

Plasmid

#71377

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Grp1-PH (Cyth3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-TmCA-P(11)4

Plasmid

#185783

Purpose

Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

TmCA-P(11)4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET-mBoNT/F

Plasmid

#168015

Purpose

Expresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

MBP-BoNT/F-LC(1-428)-6xHis

Use

Tags

Hisx6 and maltose binding protein

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET3a-hUb

Plasmid

#61937

Purpose

Expression of hUb in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

human ubiquitin (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET29b-ClbP

Plasmid

#48244

Purpose

Untagged, full-length protein for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET22b-csgB

Plasmid

#89582

Purpose

Express csgB curli protein with GS linker and Histaq

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgB (csgB )

Use

Tags

3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

Expression

Bacterial

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b-hnRNP-F

Plasmid

#23021

Depositor

Douglas Black

Article

Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

Insert

hnRNP-F (HNRNPF Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
RalGDS-YPet

Plasmid

#134929

Purpose

Fluorescently tagged affinity reagent

Depositor

Klaus Hahn

Article

O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

Insert

RaIGDS RBD, YPet

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pYpet-FsaA

Plasmid

#98279

Purpose

mammalian expression of Ypet-FsaA

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FsaA

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-I-SceI

Plasmid

#165041

Purpose

Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

I-SceI

Use

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A (S-A)

Plasmid

#8825

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A inactive (GZMA Human)

Use

Tags

(His)6

Expression

Bacterial

Mutation

changed Serine 184 to Alanine

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only