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Plasmid#104588PurposeAAV vector expressing Myc-tagged SpCas9 under the EFS promoterDepositorInsertStreptococcus pyogenes Cas9
UseAAV and CRISPRTagsMycExpressionMammalianMutationPromoterEFSAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m3
Plasmid#149556PurposeGateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMsDepositorInsertecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
1137B=Hsp70Bb-Cas9
Plasmid#153284PurposeThe attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.DepositorInsertpHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265H
Plasmid#149554PurposeGateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMsDepositorInserthAID-ziSpyMacCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266B
Plasmid#149555PurposeGateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMsDepositorInsertziSpyMacCas9(D10A)-PmCDA1-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PE7-2A-BSD
Plasmid#234073PurposeFor lentiviral expression of PE7 with blasticidin resistanceDepositorInsertPE7
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF-1α core promoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pS648∙CsR
Plasmid#197860PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS548∙CsR
Plasmid#197859PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Tc resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
ePPEplus
Plasmid#205241PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pePEmax-pPuro
Plasmid#215688PurposeCloning vector for Uni-Vector prime editing systemDepositorInsertPEmax
UseUnspecifiedTagsExpressionMutationPromoterEFS promoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#206994PurposePlasmid expressing both SpCas9 and SaCas9DepositorUseTagsP2AExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSP1594
Plasmid#65775PurposeHuman expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLSDepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pS848∙CsR
Plasmid#199240PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9-native
Plasmid#129392PurposeDerived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.DepositorInsertS. pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationAspartate 10 to Alanine, Histidine 840 to AlaninePromoterPrhaBADAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
ePPE-V223A
Plasmid#205243PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertSV40_NLS-nCas9-NC-MLV-SV40_NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]
Plasmid#149426PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PEmax-2A-BSD
Plasmid#234071PurposeFor lentiviral expression of PEmax with blasticidin resistanceDepositorInsertPEmax
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF-1α core promoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only