Addgene

We narrowed to 1,427 results for: Streptococcus

Showing: 841 - 860 of 1427 results

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AV13_pCAG.Cas9.gRNA.NT

Plasmid

#199260

Purpose

Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
Non-targeting (NT) control gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG promoter and RNA polymerase III promoter for …

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJCC_164 SpCas9 R1333A/R1335A

Plasmid

#179528

Purpose

For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 R1333A/R1335A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginines 1333 and 1335 to alanine

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_103 SpCas9 T1337C

Plasmid

#179525

Purpose

For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 T1337C

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed threonine 1337 to cysteine

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Initial Gly

Plasmid

#128685

Purpose

Expresses GB1 mutant with no Methionine residues

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Ile6Met

Plasmid

#128687

Purpose

Expresses GB1 Ile6Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Val29Met

Plasmid

#128688

Purpose

Expresses GB1 Val29Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Ala34Met

Plasmid

#128689

Purpose

Expresses GB1 Ala34Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Val39Met

Plasmid

#128690

Purpose

Expresses GB1 Val39Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Val54Met

Plasmid

#128691

Purpose

Expresses GB1 Val54Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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EC2_2_dCas9_VP64_sgRNA

Plasmid

#163707

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VP64+SV40 NLS

Use

Tags

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJT140_GalL_cCDA1-VRERBE3

Plasmid

#145103

Purpose

Expressing base editor cCDA1-VRERBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-VRERBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

Promoter

GalL

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT46_GalL_cCDA1-BE3

Plasmid

#145039

Purpose

Expresses cCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

cCDA1-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT176_GalL_eA3A-BE3

Plasmid

#145125

Purpose

Expressing base editor eA3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(N57G)-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

A3A(N57G); spCas9(D10A)

Promoter

GalL

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT171_GalL_A3AΔ178-BE3

Plasmid

#145121

Purpose

Expressing base editor A3AΔ178-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ178-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

A3A(1-178AA); spCas9(D10A)

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT170_GalL_A3AΔ182-BE3

Plasmid

#145120

Purpose

Expressing base editor A3AΔ182-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ182-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

A3A(1-182AA); spCas9(D10A)

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT169_GalL_A3AΔ186-BE3

Plasmid

#145119

Purpose

Expressing base editor A3AΔ186-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ186-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

A3A(1-186AA); spCas9(D10A)

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT166_GalL_A3A-BE3

Plasmid

#145116

Purpose

Expressing base editor A3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT139_GalL_nCDA1-VRERBE3

Plasmid

#145102

Purpose

Expressing base editor nCDA1-VRERBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-VRERBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT130_GalL_nCDA1-NGBE3

Plasmid

#145094

Purpose

Expressing base editor nCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-NGBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT122_GalL_cCDA1-VQRBE3

Plasmid

#145087

Purpose

Expressing base editor cCDA1-VQRBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-VQRBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A, D1135V, R1335Q, T1337R)

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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