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Plasmid#138215PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.DepositorInsertsACt-mCherry-NES (Adcy10 Rat)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC+] (AVA2626)
Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA-E11)2
Plasmid#123151PurposeMercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL838
Plasmid#70680PurposeThis vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-sACt-mCherry
Plasmid#138216PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.DepositorInsertLyn-sACt-mCherry (Adcy10 Rat)
UseTagsN-terminal targeting sequence from Lyn kinase and…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFIN-EF1a-GFP:2A:CHER(HA)-WPRE
Plasmid#44597DepositorInsertEF1a-GFP:2A:CHER(HA)
UseLentiviralTagsExpressionMutationPromoterhuman elongation factor -1a (pos 2528-3971)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-NRBF2 (1-159)
Plasmid#99331PurposeMammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complexDepositorInsertNuclear receptor-binding factor 2 (NRBF2 Human)
UseTags6xHis-MBP-TEVExpressionBacterialMutationAA1-159 are presentPromoterT7Available sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEE005
Plasmid#176762PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert101
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE031
Plasmid#176788PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert308
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE011
Plasmid#176768PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert203
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE012
Plasmid#176769PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert204
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE013
Plasmid#176770PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert205
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE014
Plasmid#176771PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert206
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE015
Plasmid#176772PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert207
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE016
Plasmid#176773PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert208
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE017
Plasmid#176774PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert209
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE018
Plasmid#176775PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert210
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE019
Plasmid#176776PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert211
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE020
Plasmid#176777PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert212
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only