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Plasmid#108697PurposeFor expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cellsDepositorInsertRNASEH2B (RNASEH2B Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-mCherry
Plasmid#26976PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitationDepositorHas ServiceAAV5, AAV8, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGR420
Plasmid#213782PurposeIntegrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flankDepositorInsertMET15
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGR481
Plasmid#213783PurposeIntegrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutationsDepositorInsertura3*
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-mCherry
Plasmid#108880PurposeExpression of human histone2B fused to mCherry (nuclear marker)DepositorInsertH2B-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
ampkar
Plasmid#35097PurposeA fluorescent reporter of AMPK activity and cellular energy stressDepositorInsertampkar
UseTagsECFP and VenusExpressionBacterialMutationnaPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TetON-3G
Plasmid#96963PurposeMammalian expressiom of Clontech rtTA driven by human beta-actine promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP1830
Plasmid#222728PurposepNP561 - T5 Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMCL1
Plasmid#178203PurposeAn improved vector for BacMam systemDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MGAT1-GFP
Plasmid#163647PurposeExpresses GFP tagged MGAT1 in mammalian cellsDepositorInsertalpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMA122 - peel-1 negative selection
Plasmid#34873PurposeNegative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegansDepositorInsertpeel-1 (peel-1 Nematode)
UseWorm targetingTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLPCX roGFP2-Orp1
Plasmid#64991PurposeMammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)DepositorInsertOrp1 (HYR1 Budding Yeast)
UseRetroviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hOCT3/4-shp53-F
Plasmid#27077PurposeIntegration-free (episomal) expression of human OCT3/4 and shRNA against p53DepositorInsertOCT3/4 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pOPINB-OTULIN (full-length, aa 1-352)
Plasmid#61464PurposeExpresses human OTULIN (full-length) in E. coli.DepositorInsertOTULIN (OTULIN Human)
UseTagsHis6-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR1
Plasmid#116740PurposeLentiviral expression of FGFR1DepositorInsertFGFR1 (FGFR1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EZH2
Plasmid#116738PurposeLentiviral expression of EZH2DepositorInsertEZH2 (EZH2 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
p156
Plasmid#186179PurposeShotgun Metagenomic Spike-inDepositorInsertsynDNA 156
UseSynthetic Biology and Unspecified; CloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only