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Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only
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pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tip alpha (28-192)
Plasmid#23158DepositorInsertTNF-α-inducing protein
UseTagsHisExpressionBacterialMutationdeleted amino acids 1-27PromoterAvailable sinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
mGDF8 donor mCherry-PA
Plasmid#72596PurposePlasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.DepositorInsertmCherry-puromycin cassetter
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 Sox4 mut 3'UTR oligo
Plasmid#26988DepositorInsertSox4 mut UTR oligo (SOX4 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianMutationbp 449-509 of SOX4 3'UTR; mut @ bp 483PromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
p-1459-adcy4-Luc
Plasmid#28184DepositorInsertAdcy4 promoter (Adcy4 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
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HCKan-P-TEF2
Plasmid#65337Purposeconstruction of TEF2 promoter in HCKan-P receiving vectorDepositorInsertTEF2 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-Linker_PCNA-MutC
Plasmid#98272Purposefor low level expression of mEos2-PCNA in mammalian cellsDepositorInsertPCNA (PCNA Human)
UseTagsmEos2ExpressionMammalianMutationSilent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…PromoterCMVAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
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HCKan-O-ycrtYB
Plasmid#65346Purposeconstruction of ycrtYB gene in HCKan-O receiving vectorDepositorInsertycrtYB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-O-ycrtE
Plasmid#65344Purposeconstruction of ycrtE gene in HCKan-O receiving vectorDepositorInsertycrtE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-O-ycrtI
Plasmid#65345Purposeconstruction of ycrtI gene in HCKan-O receiving vectorDepositorInsertycrtI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-SpeI
Plasmid#87331PurposeExpression of shRNA targeting at Nup93DepositorInsertshRNA against human Nup93
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac-LacI-LAP
Plasmid#114034PurposeProduction of baculovirus with LacI-LAPDepositorInsertLacI-LAP
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSALECTNK-LGK/csTEM1
Plasmid#81164PurposepSALECT plasmid with levoglucosan kinase fused to TEM-1DepositorInsertLevoglucosan kinase
UseTagsCodon swapped TEM-1 beta-lactamase (H26 to W290) …ExpressionBacterialMutationPromoterlacAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD8
Plasmid#112820PurposepSMF2 with 8 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD10
Plasmid#112821PurposepSMF2 with 10 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only