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Plasmid#176741PurposeExpresses membrane bound biotin ligase, BioID2, in astrocytesDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
ETS2_pet28a
Plasmid#131664PurposeBacterial expression of ETS gene ETS2DepositorInsertETS2 (ETS2 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET 2C-T10-dCasX
Plasmid#126418PurposeE. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purificationDepositorInsertDpbCasX
UseCRISPRTagsHis-MBPExpressionBacterialMutationPromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMBEC2
Plasmid#187596PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJA2
Plasmid#19492DepositorInsertDNAJA2 (DNAJA2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOPS0381
Plasmid#133231PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-nsp7-FLAG
Plasmid#201027PurposeExpresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.DepositorInsertnon-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTags8xHis and FLAGExpressionMammalianMutationPromoterT7 promoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsEDC3
Plasmid#25032DepositorInsertEDC3 (EDC3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-OC43-nsp12
Plasmid#168949PurposeGateway-compatible Entry vectorDepositorInsertHCoV-OC43-nsp12 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-NL63-nsp12
Plasmid#168927PurposeGateway-compatible Entry vectorDepositorInsertHCoV-NL63-nsp12 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax-NRTH
Plasmid#136922PurposeExpresses ABEmax-NRTH in mammalian cellsDepositorInsertABEmax-NRTH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis-parallel1-NLSH2BCas9
Plasmid#112065PurposeExpression and purification of NLSH2BCas9 for fungal gene editingDepositorInsertNLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
UseCRISPRTagsHis tagExpressionBacterialMutationCas9 was codon optimized for Aspergillus nigerPromoterpromoter in vectorAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJB1
Plasmid#19549DepositorInsertDnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-SPASTIN
Plasmid#180647PurposeLentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, has siRNA resistance to GAACAGUGUG…PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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EGFP-N-Ezrin
Plasmid#202684Purposefluorescent protein tagged ezrinDepositorInsertMus musculus EZR(1-586):EGFP (Ezr Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKAcat-mCherry
Plasmid#181841PurposemCherry-tagged PKA catalytic subunit for mammalian expression.DepositorInsertPKAcat-mCherry (PRKACA Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-OC43-nsp1
Plasmid#168931PurposeGateway-compatible Entry vectorDepositorInsertHCoV-OC43-nsp1 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-HKU1-nsp1
Plasmid#168893PurposeGateway-compatible Entry vectorDepositorInsertHCoV-HKU1-nsp1 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only