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Plasmid#195027PurposeHA-tagged Creb5(T59/T61A) in pInducer20 lentivirus destination vectorDepositorInsertHA-tagged Creb5 (full length, 508aa; with T59/T61A mutation) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationT59/T61APromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-iCreb5(T59/T61D)(HA)
Plasmid#195028PurposeHA-tagged Creb5(T59/T61D) in pInducer20 lentivirus destination vectorDepositorInsertHA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationT59/T61DPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1–eGFP
Plasmid#211552PurposeA piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP
Plasmid#212795PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R/K63R)-GFP
UseIntegrative vectorTagsExpressionYeastMutationCUb(K48R/K63R)PromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
p Pcrg1: Khd4-Ada-Gfp (CbxR)
Plasmid#203734PurposePlasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic domain (Ada) with three repeats of GGGGS linker in between, folDepositorInsertKhd4-Ada-Gfp
UseTagsGfpExpressionBacterialMutationPromoterPcrg1Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tev-IntC-P53(304-393)
Plasmid#200315Purposebacterial expression of P53 TETCTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
UseTagsHis6GB1tev and designed intein CExpressionBacterialMutationPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-P66KP71Q-GB1_AD
Plasmid#148516PurposeBacterial Expression of DmThor_50-83-P66KP71QDepositorInsertDmThor_50-83-P66KP71Q (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-del76-84_Z
Plasmid#148190PurposeBacterial Expression of DmThor-del76-84DepositorInsertDmThor-del76-84 (Thor Fly)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-del76-84-Y54AM59A_Z
Plasmid#148191PurposeBacterial Expression of DmThor-del76-84-Y54AM59ADepositorInsertDmThor-del76-84-Y54AM59A (Thor Fly)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-CLL78AAA-V5His6_Z
Plasmid#148165PurposeInsect Expression of DmThor-CLL78AAADepositorInsertDmThor-CLL78AAA (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-RGTtoAAA-V5His6_Z
Plasmid#148166PurposeInsect Expression of DmThor-RGTtoAAADepositorInsertDmThor-RGTtoAAA (Thor Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-Y54AM59A-V5His6_Z
Plasmid#148167PurposeInsect Expression of DmThor-Y54AM59ADepositorInsertDmThor-Y54AM59A (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-del76-84-V5His6_Z
Plasmid#148152PurposeInsect Expression of DmThor-del76-84DepositorInsertDmThor-del76-84 (Thor Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-del1047-1063-GSG-dsRNAres_Y
Plasmid#148070PurposeInsect Expression of DmPAN2-del1047-1063-GSG-dsRNAresDepositorInsertDmPAN2-del1047-1063-GSG-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationone silent mutation compared the the sequence gei…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-del76-85-V5His6_T
Plasmid#147613PurposeInsect Expression of DmThor-del76-85DepositorInsertDmThor-del76-85 (Thor Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HseIF4G_571-676-GB1_AG
Plasmid#148745PurposeBacterial Expression of HseIF4G_571-676DepositorInsertHseIF4G_571-676 (EIF4G1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HseIF4G_592-653_AG
Plasmid#148746PurposeBacterial Expression of HseIF4G_592-653DepositorInsertHseIF4G_592-653 (EIF4G1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNkx2-1#1/Cre
Plasmid#193221PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Nkx2-1 geneDepositorInsertsgNkx2-1#1 (Nkx2-1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676-GB1_AD
Plasmid#148512PurposeBacterial Expression of DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676DepositorInsertDmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNotch2#1/Cre
Plasmid#193225PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 geneDepositorInsertsgNotch2#1 (Notch2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only