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Plasmid#199367PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOEu-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9
Plasmid#84240PurposeExpresses GA-inducible VPR-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax-NRCH
Plasmid#136923PurposeExpresses ABEmax-NRCH in mammalian cellsDepositorInsertABEmax-NRCH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR357.10
Plasmid#125106PurposeExpression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)131
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FEV_pet28a
Plasmid#131668PurposeBacterial expression of ETS gene FEVDepositorInsertFEV (FEV Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-229E-Nucleocapsid
Plasmid#168967PurposeGateway-compatible Entry vectorDepositorInsertHCoV-229E-Nucleocapsid (N )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-OC43-Nucleocapsid
Plasmid#168947PurposeGateway-compatible Entry vectorDepositorInsertHCoV-OC43-Nucleocapsid (EYW02_gp8 )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
[pSK468] pLJC5 Flag-Depdc5
Plasmid#109337PurposeLenti-viral expression of Flag-tagged wildtype Depdc5DepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX ShcD-SH2
Plasmid#46488DepositorInsertShcD (SHC4 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 512-623PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75154PurposeNuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMBEC6
Plasmid#187598PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK
Plasmid#25033DepositorInsertRck (DDX6 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEntry_MERS-CoV-Nucleocapsid
Plasmid#168833PurposeGateway-compatible Entry vectorDepositorInsertMERS-CoV-Nucleocapsid (N )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sgBFP
Plasmid#192929PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2DepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-NES-L2
Plasmid#127351Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
CYFIP2.mCherry
Plasmid#122052PurposeExpresses human CYFIP2 in mammalian cells.DepositorInsertCYFIP2 (CYFIP2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 barr2 HA
Plasmid#14692DepositorInsertbeta-arrestin 2 (Arrb2 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
ATG9A-2STrep
Plasmid#210359PurposeExpresses ATG9A fused with 2Strep in mammalian cellsDepositorInsertATG9A (ATG9A Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES_mScarleti_Sec23A
Plasmid#117273PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23ADepositorInsertsUseTagsmScarlet-iExpressionMammalianMutationHuman Sec23A; Sequencing showed a mutation in Sec…PromoterCMV and IRESAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only