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Plasmid#47730PurposeExpresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised PF3D7_1136200
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin replaced…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Flrt3-Fc-His
Plasmid#72075PurposeExpresses the extracellular region of the FLRT3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertFlrt3 (Flrt3 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-EphB4-SV40-GFP (B4G)
Plasmid#65444PurposeExpression of human EPHB4 and GFP in mammalian cellsDepositorInsertEPHB4 (EPHB4 Human)
UseLentiviralTagsSV40-EGFPExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATP416-pluxO5-pphlO6-crtYBI
Plasmid#165977PurposeExpresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pluxO5), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Neuroligin-2 scFv [L107/90]
Plasmid#206763PurposeMammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/90 scFv.DepositorInsertNeuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMG024 MBP-GSK3β-HA-His, GST_λPPase
Plasmid#196182PurposeCo-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-HisDepositorInsertsUseTagsGST, HA, His6, and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUS-WT-VC
Plasmid#175068Purposeexpresses FUS wildtype tagged to mVenus and mCherry in mammalian cellsDepositorInsertFUS glycine rich protein (FUS Human)
UseTagsHA and mVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATP416-ptetO7-pphlO6-crtYBI
Plasmid#165976PurposeExpresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pphlO6), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AR-LBD
Plasmid#89111Purposemammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tagDepositorInserthAR ligand binding domain (AR Human)
UseTags2xHAExpressionMammalianMutationcontains Ligand Binding Domain (624-919)PromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dscaml1-Fc-His
Plasmid#72072PurposeExpresses the extracellular region of the Dscaml1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscaml1 (Dscaml1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Anti-Nav1.1 Na+ channel [K74/71R]
Plasmid#128626PurposeMammalian Expression Plasmid of anti-Nav1.1 Na+ channel (Rat). Derived from hybridoma K74/71.DepositorInsertanti-Nav1.1 Na+ channel (Rat) recombinant mouse monoclonal antibody (Scn1a Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Parvalbumin scFv [L114/3]
Plasmid#182072PurposeMammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/3.DepositorInsertrecombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T-1 GST-Ago2 S387A
Plasmid#60656PurposeExpresses GST-Ago2 S387A mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsGSTExpressionBacterialMutationS387APromotertacAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC1GFP-Kir7.1
Plasmid#79118PurposeExpress N-terminal fused Kir7.1 protein in mammalian cellsDepositorInsertInwardly rectifying potassium channel KCNJ13 (KCNJ13 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Anti-VGlut2 [N29/29R]
Plasmid#114501PurposeMammalian Expression Plasmid of anti-VGlut2 (Rat). Derived from hybridoma N29/29.DepositorInsertanti-VGlut2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a6 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP mScarlet-ER [TM(SAC1)]
Plasmid#186572PurposeExpression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cellsDepositorInsertSAC1 (SACM1L Human)
UseRetroviralTagsEGFPExpressionMammalianMutationC-terminal transmembrane region of the protein: 5…PromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Neurexin-1-Beta [N170A/1.1R]
Plasmid#114530PurposeMammalian Expression Plasmid of anti-Neurexin-1-Beta (Human). Derived from hybridoma N170A/1.1.DepositorInsertanti-Neurexin-1-Beta (Homo sapiens) recombinant mouse monoclonal antibody (NRXN1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
NCKX4 scFv [N414/25]
Plasmid#190553PurposeMammalian Expression of NCKX4 scFV. Derived from hybridoma N414/25.DepositorInsertNCKX4 (Homo sapiens) recombinant scFV (Slc24a4 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Cep170 C-term (Nigg GG2)
Plasmid#41151DepositorInsertCep170 (CEP170 Human)
UseTagseGFPExpressionMammalianMutationContains C-terminal fragment (aa 755-1460, from X…PromoterCMV, T7Available sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Anti-Co-Rest/RCOR1 [K72/8R]
Plasmid#140066PurposeMammalian Expression Plasmid of anti-Co-Rest/RCOR1 (Human). Derived from hybridoma K72/8.DepositorInsertanti-Co-Rest/RCOR1 (Human) recombinant mouse monoclonal antibody (RCOR1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only