Addgene

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Gly-tRNA-gRNA scaffold for library 2

Plasmid

#192365

Purpose

tRNA sequence provider (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gly tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Pro-tRNA-gRNA scaffold for library 2

Plasmid

#192366

Purpose

tRNA sequence provider (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Pro tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gln-tRNA-gRNA scaffold for library 1&2

Plasmid

#192367

Purpose

tRNA sequence provider (for checkpoint and immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gln tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gly-tRNA-gRNA scaffold for library 1

Plasmid

#192368

Purpose

tRNA sequence provider (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gly tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Pro-tRNA-gRNA scaffold for library 1

Plasmid

#192369

Purpose

tRNA sequence provider (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Pro tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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DpbCas12e

Plasmid

#188495

Purpose

Expresses FLAG tagged DpbCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12j2

Plasmid

#188498

Purpose

Expresses FLAG tagged Cas12j2

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12j2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PlmCas12e

Plasmid

#188496

Purpose

Expresses FLAG tagged PlmCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e KRAB

Plasmid

#188505

Purpose

Expresses FLAG tagged DpbdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12j3

Plasmid

#188497

Purpose

Expresses FLAG tagged Cas12j3

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12j3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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p-preSufI(IAC)

Plasmid

#168516

Purpose

Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

pre-SufI(IAC)

Use

Tags

6xHisC tag and HSV tag

Expression

Bacterial

Mutation

C17I and C295A

Promoter

T7

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_mcherry

Plasmid

#167894

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_mcherry

Plasmid

#167870

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
CRISPR_HLA_DPB

Plasmid

#164989

Purpose

Expression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DPB1*01:01:01

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DQB

Plasmid

#164991

Purpose

Expression of gRNA targeting HLA-DQB locus, including DQB1*05:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DQB1*05:01:01

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DQA

Plasmid

#164990

Purpose

Expression of gRNA targeting HLA-DQA locus, including DQA1*01:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DQA1*01:01:01

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LPLKMLNI_epitope

Plasmid

#165015

Purpose

Expression of CMV UL83 (116-123) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL83 (116-123)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMA5460

Plasmid

#184855

Purpose

Encodes G418 resistance and PhiC31-excisable hTFAM gene

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

TFAM (TFAM Human)

Use

Retroviral; Phic31 attp-attb recombination/excisi…

Tags

Expression

Mammalian

Mutation

WT

Promoter

LTR

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMA4000

Plasmid

#184857

Purpose

Cre-excisable TFAM in Blasticidin-resistannce retroviral vector

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

TFAM (TFAM Human)

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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KBB345

Plasmid

#185078

Purpose

rfa3D46 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA3

Use

Tags

GFP

Expression

Yeast

Mutation

Rfa3 truncation aa 1-46

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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