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Plasmid#138569PurposeUniversal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJDepositorInsertClover-BlastR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W
Plasmid#32520DepositorInsertConstitutively active Rheb (S16H) (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationS16H to make constitutively activePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionTagsExpressionMutationPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT
Plasmid#173943PurposeBacterial expression of isolated PARP1 CAT domainDepositorInsertPARP1-CAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Nucbow
Plasmid#158992PurposeMulticolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS2E
Plasmid#55169PurposeEmpty vector, integration at lacA, ampr, mlsrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry
Plasmid#139649PurposeReporter assayDepositorInsertH2B-mCherry
UseLentiviralTagsFLAG, His6ExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1
Plasmid#194278PurposeExpresses gRNA and dSaCas9-KRAB from lentiviral vectorDepositorInsertshumanized dSaCas9 KRAB T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhU6 and hUbCAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBXNPHM3
Plasmid#110099PurposeE.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage siteDepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBMN-HA-TBK1
Plasmid#199205PurposeUsed for the expression of TBK1 (SMC Internal No. 1996)DepositorInsertTBK1 (TBK1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pyc111s
Plasmid#158781Purposeribosome-tagged, Cre-dependent GCaMP for soma localizationDepositorInsertGCaMP6s-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL636
Plasmid#189830PurposeThe T-DNA backbone of Cas12j2 for transcriptional activation in riceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterpZmUbi1Available sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL638
Plasmid#189831PurposeThe T-DNA backbone of nCas12j2 for genome editing in riceDepositorTypeEmpty backboneUseCRISPR; T-dna cloneTagsExpressionMutationPromoterZmUbi1Available sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_Zeo
Plasmid#118596PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SspB-mScarlet-I-MYPT169
Plasmid#178522PurposeExpresses SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cellsDepositorInsertSspB-mScarlet-I-MYPT169
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP-SaCas9-HF (without sgRNA)
Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Fbx15-cont2
Plasmid#27081DepositorInsertFBXO15 (FBXO15 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Xpress-GBF1
Plasmid#206417PurposeMammalian expression of hGBF1DepositorInsertGBF1 (GBF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-cmv-SpCas9-TadDE-BlastR
Plasmid#193850PurposeExpress TadDE in mammalian cells with BlastR selection, Tol2 integrationDepositorInsertTadDE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIG PIK3CA E545K
Plasmid#73055PurposeHuman PIK3CA E545KDepositorInsertPIK3CA (PIK3CA Human)
UseTagsExpressionMammalianMutationE545KPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only