-
Plasmid#218713PurposeExpresses N-terminally EGFP-tagged BBS4 in mammalian cellsDepositorInsertBBS4 (BBS4 Human)
UseTagsEGFPExpressionMammalianMutationI354T (t1110c)PromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLO 62.4
Plasmid#123123PurposeHuman expression vector for deltaproteobacteria CasX and sgRNADepositorInsertsCasX protein
CasX sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
Plasmid#187336PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertdCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-UbxD8
Plasmid#53777Purposeexpress FLAG-tagged human UbxD8 geneDepositorInsertUbxD8 (FAF2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL0812 (pCRISPR(BbsI)_Vch)
Plasmid#200898PurposeExpresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.DepositorInsertVchCRISPR (entry)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Brm WT-Flag pShuttle
Plasmid#122105PurposeExpression of full length Brm in mammalian cellsDepositorInsertBRM (SMARCA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN479
Plasmid#86234PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locusDepositorInsertspCas9-nickase and sgRNA against mouse rosa26 locus
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH2-B-SH2
Plasmid#46479DepositorInsertSH2-B (SH2B1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 515-639PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP VPS16-GFP
Plasmid#67023PurposeHuman VPS16 ORF was inserted into pMRXIP GFP-N vectorDepositorInsertHomo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)
UseRetroviralTagsGFPExpressionMutationPromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-MCU-GFP
Plasmid#31732DepositorInsertMCU (MCU Human)
UseTagsGFPExpressionMammalianMutation*PromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75E)
Plasmid#137829PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75E)
UseTags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Glutamic Acid in NTEVp fr…PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Med29 pBacPAK8
Plasmid#15369DepositorInsertMed29 (MED29 Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
pET30a-SARS-CoV2-NP
Plasmid#165090Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5/FRT/TO GFP DNAJA1
Plasmid#19491DepositorInsertDNAJA1 (DNAJA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMK1253
Plasmid#133058PurposeLentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.DepositorInsertTau.K18(LM)
UseLentiviralTagsmClover2ExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-Mau2_Neo_FKBPF36V_mScarlet
Plasmid#163881PurposeAcute Depletion of Mau2-mScarletDepositorInsertMau2 (Mau2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn3
Plasmid#164959PurposeZebrafish kcnn3 gene CDSDepositorInsertkcnn3 (kcnn3 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-1
Plasmid#72680PurposeSame as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationlower strength RBS before mutL E32K allele than i…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
hRAD18-EGFP
Plasmid#68824PurposeExpresses human RAD18 tagged with EGFPDepositorInserthuman RAD18 tagged with EGFP (RAD18 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only