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FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

Plasmid

#216172

Purpose

Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- Control- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

Plasmid

#216173

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA

Plasmid

#220223

Purpose

AAV-STARR-seq validation control vector with TFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 WT1 +1 ATG

Plasmid

#217643

Purpose

Dox inducible WT1 cDNA - as transcribed from +1ATG (long isoform)

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 long isoform (WT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 WT1 +378 ATG

Plasmid

#217644

Purpose

Dox inducible WT1 cDNA - as transcribed from +378ATG (short isoform)

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 short isoform (WT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 Ex8 mut WT1

Plasmid

#217645

Purpose

Dox inducible WT1 cDNA with exon 8 mutation

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 (WT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Frameshift exon 8

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 Ex9 mut WT1

Plasmid

#217646

Purpose

Dox inducible WT1 cDNA with exon 9 mutation

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 (WT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Frameshift exon 9

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4293

Plasmid

#200161

Purpose

Ptwk-40s GCaMP6s::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 mNeptune

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GCaMP6

Use

Tags

mNeptune

Expression

Worm

Mutation

Promoter

Ptwk-40

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4506

Plasmid

#200255

Purpose

Pnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

TeTx

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4508

Plasmid

#200256

Purpose

Pflp-18 LoxP EBFP LoxP TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

TeTx

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Pflp-18

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH2673

Plasmid

#199691

Purpose

Pnmr-1 FLPe unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of FLPe

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

flpE (flpE )

Use

Tags

no

Expression

Worm

Mutation

Promoter

Pnmr-1 long

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2829

Plasmid

#199693

Purpose

Pcex-1 tomm-20::miniSOG UrSL mCherry unc-54 3' UTR C.elegans RIM neuron expression of miniSOG RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

miniSOG

Use

Tags

RFP

Expression

Worm

Mutation

Promoter

Pcex-1

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-GST-TEV-NAP1

Plasmid

#217610

Purpose

For expression in E. coli of NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

TAC promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-GST-TEV-GFP-NAP1

Plasmid

#208870

Purpose

For expression in E. coli of GFP-NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Use

Tags

GFP and GST-TEV

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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M2e-noro-VLP

Plasmid

#201192

Purpose

Expresses noro-VLP decorated with influenza M2e in insect cells

Depositor

Vesa Hytönen

Article

Lampinen et al Front Cell Infect Microbiol. 2023 Jun 22

Insert

M2e-noro-VLP

Use

Tags

Influenza M2e peptide

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-mCherry-FLAG

Plasmid

#212798

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Tags

mCherry-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-FLAG

Plasmid

#212797

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R/K63R)-yeGFP-His6

Plasmid

#212785

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-CUbo-mCherry-VSV

Plasmid

#212734

Purpose

Consitutive expression of CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

mCherry-VSV

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-CUbo-VSV

Plasmid

#212733

Purpose

Consitutive expression of CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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