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Plasmid#50807PurposeExpresses enzymatically monobiotinylated full length MSP8 ectodomainDepositorInsertMSP8
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
PHMcc(delta16 PHM 356s)
Plasmid#25455DepositorInsertPHM (Pam Rat)
UseTagsExpressionMammalianMutationContains the catalytic core of PHM, including aa …PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHL 1916
Plasmid#53023PurposeAmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertfimB
UseSynthetic BiologyTagsAsp terminator and RBS(st7)ExpressionBacterialMutationPromoterpLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A3T
Plasmid#62260Purposeexpression of A3T sgRNA from the arabinose-inducible promoterDepositorInsertA3T sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A3NT
Plasmid#62259Purposeexpression of A3NT sgRNA from the arabinose-inducible promoterDepositorInsertA3NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1575
Plasmid#53028PurposePconNoHind:csrB, PLtetO-1:RBS(st3):csrDDepositorInsertscsrB
csrD
UseSynthetic BiologyTagsRBS (st3)ExpressionBacterialMutationPromoterPconNoHind and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1756
Plasmid#53036PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pConNoHindM12Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)
Plasmid#225441PurposeModule for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInserthygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5418_pMBP-GlcR
Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
UseTags10x His tag with E. coli maltose binding protein …ExpressionBacterialMutationPromoterT7 promoter and T7 promoter-lacOAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K
Plasmid#214460PurposeGateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G3 (CSNK1G3 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K
Plasmid#214458PurposeGateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G1 (CSNK1G1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK11A_IDG-K
Plasmid#214461PurposeGateway destination clone of CDK11A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK11A (CDK11A Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
NBla_1.2_GpA_G83I
Plasmid#207844PurposeBLaTM-System GpA G83I negative control in NBLa 1.2 vectorDepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
NBla_1.2_GpA_wt
Plasmid#207842PurposeBLaTM-System GpA wt positive control in NBLa 1.2 vectorDepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
CBLa_1.2_GpA_wt
Plasmid#207843PurposeBLaTM-System GpA wt positive control in CBLa 1.2 vectorDepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
CBLa_1.2_GpA_G83I
Plasmid#207845PurposeBLaTM-System GpA G83I negative control in CBLa 1.2 vectorDepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
NODAL-MYC-DYK-N72A-N199A
Plasmid#115252PurposeFor mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsMYC-DYKExpressionMammalianMutationN72A, N199APromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK-N328A
Plasmid#115246PurposeFor mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tagDepositorInsertNODAL splice variant (NODAL Human)
UseTagsMYC-DYKExpressionMammalianMutationN328APromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-pntAB
Plasmid#158609PurposeLow phosphate induction of pntABDepositorInsertpntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)
UseTagsExpressionBacterialMutationPromoterInsulated ugpBp from E. coliAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC016.109
Plasmid#171144PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only