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pPK1E-1-gfp
Plasmid#48133PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
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MDH1-PGK-FLAG-TAT-86-E
Plasmid#35978DepositorInserttransactivating protein (tat Human Immunodeficiency Virus)
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationFrom the "E" HIV cladePromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pSLQ1371-sgYAP-2
Plasmid#121423PurposesgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgYAP-2
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Sa sgRNA expression vector
Plasmid#107720PurposeFor in vitro transcription of Sa sgRNA from the T7 promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52543PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attP (full length)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAP2050-2
Plasmid#105243Purposemammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linkerDepositorInsertGFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI Flag Smad3 NL WT
Plasmid#27040DepositorInsertSmad3 (SMAD3 Human)
UseTagsFlagExpressionMammalianMutationaa 1-226PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52547PurposeTargeting vector for homologous recombination in mouse ES cells; Rosa26 locusDepositorInsertsPhiC31o attP (full length)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Flag Smad3 EPSM A204S
Plasmid#27111DepositorInsertSmad3 (SMAD3 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationT179V S204A S208A S213A; A204SPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FUS_C2G_R521G
Plasmid#26369DepositorInsertFUS (FUS Human)
UseEntry vectorTagsExpressionMutationR521GPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
LEPG_shSik3.3974
Plasmid#125855PurposeRetroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance geneDepositorInsertshRNA targeting Sik3
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
LEPG_shSik3.308
Plasmid#125878PurposeRetroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance geneDepositorInsertshRNA targeting Sik3
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAP2042-1
Plasmid#105244Purposemammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linkerDepositorInserteGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-Smad3 EPSM A204S
Plasmid#27108DepositorInsertSmad3 (SMAD3 Human)
UseTagsGSTExpressionBacterialMutationT179V S204A S208A S213A; A204SPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMflPT-o4
Plasmid#101315PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
pac resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflST-o4
Plasmid#101316PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
aadA1 resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107322PurposeExpresses SpCas9 (R1335K) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) is fused to SaCas9PromoterCMV IE93Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only
