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Plasmid#221081PurposeSEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2w/b
Plasmid#111568PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorUseAAVTagsmCherryExpressionMutationPromoterAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-cGASsh4
Plasmid#127646PurposeKnock-down of human cGASDepositorInsertcGAS shRNA (CGAS Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACTN2-pEGFP
Plasmid#52669PurposeOverexpression of GFP-tagged α-actinin-2DepositorInsertactinin 2 (ACTN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGD-P1/HC-Pro
Plasmid#196328Purpose35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressorDepositorInserttobacco etch virus P1/HC-Pro
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGD-γb
Plasmid#196327Purpose35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressorDepositorInsertbarley stripe mosaic virus γb
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Ndi-1/myc-His
Plasmid#127503PurposeExpresses myc-tagged yeast Ndi-1 in mammalian cellsDepositorInsertNdi-1 (NDI1 Budding Yeast)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cilantro 2
Plasmid#74450PurposeCan clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-VC
Plasmid#110648Purposeexpresses a PAR-binding (PBZ) domain fused to venus C-terminusDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsHA and VCExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-PARP1
Plasmid#211578PurposeCMV driven PARP1-eGFP expressing construct with IRES-Neomycin selectionDepositorInsertPARP1 (PARP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-betaCatenin
Plasmid#114281PurposeTET-inducible expression of a constitutively active form of the BetaCatenin proteinDepositorInsertCatenin beta 1 (CTNNB1 Human)
UseLentiviralTagsFLAGExpressionMutationdeletion of AA 1 to 45PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PMP34-mApple
Plasmid#214417PurposePeroxisome localization of PMP34 fused to mAppleDepositorInsertPMP34 (SLC25A17 Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-H2B-V5-mScarlet
Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSV39-ugi
Plasmid#186556PurposeBroad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)DepositorInsertugi
UseTagsExpressionBacterialMutationPromoterLacUV5Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE
Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
APEX2-6xHp
Plasmid#214411PurposeLipid droplet localization of APEX2-6xHp for electron microscopyDepositorInsert6x Spastin aa 43-92 (APEX2 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mIRFP670nuc-TEV-Xph20-ETWV
Plasmid#133044PurposeExpresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporterDepositorInsertXph20-ETWV
UseTagsNLS, TEV protease + TEV cleavage site, and miRFP6…ExpressionMammalianMutationS63KPromoterCAGAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP HAT (1324-1700)
Plasmid#99340Purposebacterial expression of His tagged mouse CBP HAT domain (1324-1700)DepositorInsertmouse CBP HAT domain (1324-1700) (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
UseTagsHis6-SUMO and designed intein CExpressionBacterialMutationPromoterT7Available sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only