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Plasmid#53740PurposeFor expression of METTL14 (methyltransferase-like 14) in human cellsDepositorInsertMethyltransferase-like 14 (METTL14 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-cMyc
Plasmid#46970Purposelentiviral expression of c-MycDepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP1_Z
Plasmid#148150PurposeMammalian Expression of Hs4EBP1DepositorInsertHs4EBP1 (EIF4EBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pENTR_L1-miniTurbo-YFP-NLS-STOP-L2
Plasmid#127362Purposegateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choiceDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag HA PGC-1 alpha
Plasmid#14426DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseAdenoviralTagsFlag and HAExpressionMammalianMutationLast four amino acids of PGC-1a removed to make t…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hMALAT1
Plasmid#118580Purposeto overexpress human MALAT1 in mammalian cellsDepositorInsertMALAT1 (MALAT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_18xTEAD_luc2
Plasmid#173003Purposefirefly luciferase reporter vector with 18x clustered TEAD binding sitesDepositorInsert18xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS117 His14-Avi-45xGS-anti GFP nanobody
Plasmid#199370PurposeBacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-GFP nanobody
UseTags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
opto-Cdc42
Plasmid#174510PurposeExpresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.DepositorInsertopto-Cdc42-mCherry
UseTags3xFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH051
Plasmid#171625Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-BsmBI-Destination
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGH10 - RlucFluc minus 1 HIV-1
Plasmid#198224PurposeFluorescence reporters for -1 ribosomal frameshifting in mammalian cellsDepositorInsertsRenilla luciferase
Firefly luciferase
UseTagsExpressionMammalianMutationPromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-6xTR-TUBE
Plasmid#110313PurposeE. coli expression and purification of 6xTR-TUBEDepositorInsert6xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_G3BP1
Plasmid#127106DepositorInsertG3BP1 (G3BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nme2Cas9_AAV
Plasmid#119924PurposeAll-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassetteDepositorInsertNme2Cas9
UseAAVTagsNLS-3xHA-NLS and NLS-NLSExpressionMammalianMutationPromoterU1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-eYFP
Plasmid#117382PurposeAn AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
UseTagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV
Plasmid#216156PurposeExpresses mScarlet regardless of TDP-43 knockdown.DepositorInsertmScarlet with C-terminal FLAG tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSR357.24
Plasmid#125109PurposeExpression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only