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pCMV6-MPP8-delta55-118-10xHis

Plasmid

#194180

Purpose

Expresses MPP8-delta55-118_10xHis

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta55-118 (chromo domain deletion)

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 NSP7/bat-CoV RaTG13 C-V5-tag

Plasmid

#179974

Purpose

Mammalian expression vector for SARS-CoV-2 Nsp7 or bat RaTG13 Nsp7, V5-tagged (SARS-CoV-2 Nsp7 and RaTG13 Nsp7 have the same amino acid sequence and codon optimized sequence).

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 Nsp7 (ORF1ab Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE2 mutant

Plasmid

#133309

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE2 mutant (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

gccacatgcacc changed to gcAaAaAAcacc

Promoter

eIF3B

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron BA.2

Plasmid

#183700

Purpose

expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Tags

Expression

Mammalian

Mutation

c-terminal 18 aa deletion

Promoter

CMV

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron_BA.1

Plasmid

#180375

Purpose

Pseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike protein

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (omicron BA.1 variant) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Tags

Expression

Mammalian

Mutation

c terminal D18

Promoter

CMV

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BA.4/5

Plasmid

#186810

Purpose

pseudovirus plasmid expressing BA.4 and BA.5 spike protein

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

43740568 (S SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

HY69-70del, L452R, F486V, Q493R, on the basis of …

Promoter

CMV

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Beta

Plasmid

#177962

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )

Use

Tags

Expression

Mammalian

Mutation

K417N, E484K, N501Y, D614G

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCW-codon optimized catalytically inactive PHGDH

Plasmid

#154903

Purpose

Expresses codon optimized catalytically inactive PHGDH in mammalian cells

Depositor

Michael Pacold

Article

Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Three mutations: D175N, R236K, H283A

Promoter

TRE

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron_BA.2.75

Plasmid

#190674

Purpose

expressing SARS-CoV-2 BA.2.75 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Cell Host Microbe. 2022 Nov 4. pii: S193

Insert

Spike (S SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

K147E, W152R, F157L, I210V, G257S, D339H, G446S, …

Promoter

CMV

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Epsilon

Plasmid

#177964

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )

Use

Tags

Expression

Mammalian

Mutation

S13I, W152C, L452R, D614G

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-K417N

Plasmid

#177969

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, K417N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, K417N) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, K417N

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N439K

Plasmid

#177970

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N439K)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N439K) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, N439K

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-L452R

Plasmid

#177971

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, L452R) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, L452R

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S477N

Plasmid

#177972

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S477N) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, S477N

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N501Y

Plasmid

#177975

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, N501Y

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S13I

Plasmid

#177965

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S13I)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S13I) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, S13I

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-T20N

Plasmid

#177966

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, T20N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, T20N) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, T20N

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-P26S

Plasmid

#177967

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P26S)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, P26S) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, P26S

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-W152C

Plasmid

#177968

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, W152C)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, W152C) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, W152C

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-P681H

Plasmid

#177973

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P681H)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, P681H) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, P681H

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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